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Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development
Nagesh K Tripathi1, Ambuj Shrivastava2
1Bioprocess Scale Up Facility, Defence Research and Development Establishment, Gwalior, India.
Frontiers in Bioengineering and Biotechnology
|January 11, 2020
Summary
Advancements in recombinant DNA technology enable large-scale production of therapeutic proteins using diverse expression systems. Efficient bioprocessing strategies, including continuous manufacturing and Quality by Design, are key for developing these vital medical treatments.
Area of Science:
- Biotechnology
- Biopharmaceutical Manufacturing
- Recombinant Protein Production
Background:
- Recombinant proteins are crucial for treating diseases, vaccines, and diagnostics.
- Infectious diseases and cancers are leading global causes of mortality.
- Recombinant DNA technologies are essential for producing therapeutic proteins.
Purpose of the Study:
- To review recent developments in bioprocessing for recombinant protein production.
- To highlight advancements in expression systems and bioprocess optimization.
- To discuss upstream and downstream processing strategies for therapeutic proteins.
Main Methods:
- Review of current literature on recombinant protein bioprocessing.
- Analysis of various expression systems (prokaryotic, eukaryotic, plants).
- Examination of advanced bioprocessing techniques (continuous processing, QbD, PAT).
Main Results:
- Diverse expression systems (mammalian, microbial, plant) are utilized for recombinant protein production.
- High-throughput screening, disposable systems, and continuous processing enhance bioprocess efficiency.
- Quality by Design (QbD) and Process Analytical Technology (PAT) improve product yield and quality.
Conclusions:
- Efficient bioprocessing is critical for the industrial-scale production of therapeutic proteins.
- Integrated continuous bioprocessing offers significant advantages for manufacturing.
- Ongoing innovations in bioprocessing are vital for meeting global healthcare needs.
Keywords:
continuous chromatographydownstream processinghigh-throughput technologyintegrated continuous bioprocessingperfusion cell cultureprocess developmentprotein expressionupstream processing
