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Recombinant DNA

Recombinant DNA

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Related Experiment Video

Updated: Dec 31, 2025

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

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Published on: December 15, 2017

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development.

Nagesh K Tripathi¹, Ambuj Shrivastava²

¹Bioprocess Scale Up Facility, Defence Research and Development Establishment, Gwalior, India.

Frontiers in Bioengineering and Biotechnology

|January 11, 2020

Summary

Advancements in recombinant DNA technology enable large-scale production of therapeutic proteins using diverse expression systems. Efficient bioprocessing strategies, including continuous manufacturing and Quality by Design, are key for developing these vital medical treatments.

Keywords:

continuous chromatography downstream processing high-throughput technology integrated continuous bioprocessing perfusion cell culture process development protein expression upstream processing

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Last Updated: Dec 31, 2025

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

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Published on: December 15, 2017

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

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Published on: October 16, 2014

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

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Published on: July 23, 2013

Area of Science:

Biotechnology
Biopharmaceutical Manufacturing
Recombinant Protein Production

Background:

Recombinant proteins are crucial for treating diseases, vaccines, and diagnostics.
Infectious diseases and cancers are leading global causes of mortality.
Recombinant DNA technologies are essential for producing therapeutic proteins.

Purpose of the Study:

To review recent developments in bioprocessing for recombinant protein production.
To highlight advancements in expression systems and bioprocess optimization.
To discuss upstream and downstream processing strategies for therapeutic proteins.

Main Methods:

Review of current literature on recombinant protein bioprocessing.
Analysis of various expression systems (prokaryotic, eukaryotic, plants).
Examination of advanced bioprocessing techniques (continuous processing, QbD, PAT).

Main Results:

Diverse expression systems (mammalian, microbial, plant) are utilized for recombinant protein production.
High-throughput screening, disposable systems, and continuous processing enhance bioprocess efficiency.
Quality by Design (QbD) and Process Analytical Technology (PAT) improve product yield and quality.

Conclusions:

Efficient bioprocessing is critical for the industrial-scale production of therapeutic proteins.
Integrated continuous bioprocessing offers significant advantages for manufacturing.
Ongoing innovations in bioprocessing are vital for meeting global healthcare needs.