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Updated: Dec 31, 2025

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

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Published on: August 15, 2019

Acetogenic Fermentation From Oxygen Containing Waste Gas.

Teresa Mohr¹, Alba Infantes¹, Lars Biebinger¹

¹Technical Biology, Institute of Process Engineering in Life Science, Karlsruhe Institute of Technology, Karlsruhe, Germany.

Frontiers in Bioengineering and Biotechnology

|January 11, 2020

Summary

This study presents a two-phase microbial system for producing acetate from waste gas containing oxygen. A novel method uses bacteria to consume oxygen, enabling efficient acetate production from carbon monoxide.

Keywords:

Clostridium ljungdahlii Parageobacillus thermoglucosidasius Wood-Ljungdahl pathway anaerobic acetate production syngas water-gas shift reaction

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Area of Science:

Biotechnology
Microbial Engineering
Sustainable Chemistry

Background:

Microbial production of bulk chemicals offers a sustainable alternative to fossil fuel-based methods.
Acetogens utilize waste gases like syngas (CO, CO2, H2) for chemical synthesis.
Oxygen in feed gas inhibits acetogen activity, necessitating costly removal steps.

Purpose of the Study:

To develop a cost-effective, two-phase microbial system for acetate production from oxygen-containing waste gas.
To overcome oxygen inhibition in acetogenic fermentation using a biological approach.

Main Methods:

A two-phase system was engineered using *Parageobacillus thermoglucosidasius* and *Clostridium ljungdahlii*.
*P. thermoglucosidasius* consumed oxygen and produced H2 and CO2 in the first phase.
*C. ljungdahlii* utilized these products for acetate synthesis in the second phase.

Main Results:

The system successfully produced acetate from a gas mixture of CO and O2.
0.52 mmol of acetate was produced from 3.3 mmol of CO.
An acetate yield of 0.16 mol/mol CO was achieved, representing 63% of the theoretical maximum.

Conclusions:

The developed two-phase microbial system effectively mitigates oxygen inhibition in acetogenesis.
*P. thermoglucosidasius* serves as a biological oxygen scavenger, enabling efficient chemical production.
This approach holds potential for producing various bulk chemicals from oxygen-containing waste gases.