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MicroRNA-451b Participates in Coronary Heart Disease By Targeting VEGFA

Jie Lin1, Jun Jiang2, Ruifang Zhou2

  • 1Department of Cardiology, Taizhou People's Hospital, Taizhou 225300, China.

Open Medicine (Warsaw, Poland)
|January 11, 2020
PubMed

Insights

MicroRNA-451 (miR-451) is elevated in coronary artery disease (CAD) patients, inhibiting endothelial cell proliferation and promoting apoptosis. This suggests miR-451 could serve as a novel biomarker for CAD diagnosis and treatment.

Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Coronary artery disease (CAD) is a leading cause of global hospitalization and morbidity.
  • MicroRNAs (miRNAs) are implicated in cardiovascular disease pathogenesis.
  • The role of miR-451 in CAD remains unexplored, despite its known involvement in cancers.

Purpose of the Study:

  • To elucidate the mechanism of miR-451 in coronary artery disease (CAD).
  • To investigate the function of miR-451 in human umbilical vein endothelial cells (HUVECs).

Main Methods:

  • Analysis of miR-451 levels in peripheral blood of CAD patients.
  • Target validation using TargetScan and dual-luciferase reporter gene assays.
  • Assessment of HUVEC proliferation and apoptosis via CCK-8 and flow cytometry assays.
  • Investigation of the PI3K-Akt-mTOR signaling pathway.

Main Results:

  • miR-451 was significantly upregulated in the peripheral blood of patients with coronary artery disease.
  • VEGFA was identified as a direct target gene of miR-451.
  • miR-451 mimic suppressed HUVEC proliferation and induced apoptosis.
  • The effects of miR-451 in HUVECs were linked to the PI3K-Akt-mTOR pathway.

Conclusions:

  • miR-451 plays a critical role in the biological processes of HUVECs relevant to CAD.
  • miR-451 may serve as a potential novel biomarker for coronary artery disease.
  • Targeting miR-451 could offer a therapeutic strategy for CAD.

Keywords:
Coronary heart diseaseHUVECsPI3K-Akt-mTOR pathwayVEGFAmiR-451

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