The Effect of Gadolinium Doping in [¹³ C₆ ,² H₇ ]Glucose Formulations on ¹³ C Dynamic Nuclear Polarization at 3.35 T

The promise of hyperpolarized glucose as a non-radioactive imaging agent capable of reporting on multiple metabolic routes has led to recent advances in its dissolution-DNP (dDNP) driven polarization using UV-light induced radicals and trityl radicals at high field (6.7 T) and 1.1 K. However, most preclinical dDNP polarizers operate at the field of 3.35 T and 1.4-1.5 K. Minute amounts of Gd³⁺ complexes have shown large improvements in solid-state polarization, which can be translated to improved hyperpolarization in solution. However, this Gd³⁺ effect seems to depend on magnetic field strength, metal ion concentration, and sample formulation. The effect of varying Gd³⁺ concentrations at 3.35 T has been described for ¹³ C-labeled pyruvic acid and acetate. However, it has not been studied for other compounds at this field. The results presented here suggest that Gd³⁺ doping can lead to various concentration and temperature dependent effects on the polarization of [¹³ C₆ ,² H₇ ]glucose, not necessarily similar to the effects observed in pyruvic acid or acetate in size or direction. The maximal polarization for [¹³ C₆ ,² H₇ ]glucose appears to be at a Gd³⁺ concentration of 2 mM, when irradiating for more than 2 h at the negative maximum of the DNP intensity profile. Surprisingly, for shorter irradiation times, higher polarization levels were determined at 1.50 K compared to 1.45 K, at a [Gd³⁺ ]=1.3 mM. This was explained by the build-up time constant and maximum at these temperatures.