Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

An enhancer of DNA replication.

M L Gennaro¹, R P Novick

¹Public Health Research Institute, New York, New York 10016.

Journal of Bacteriology

|December 1, 1988

Summary

The cmp sequence enhances DNA replication in Staphylococcus aureus plasmids by improving competition for the RepC initiator protein. Its deletion significantly reduces plasmid replication frequency.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Impact of DHCWs' Safety Perception on Vaccine Acceptance and Adoption of Risk Mitigation Strategies.

JDR clinical and translational research·2022

Same author

The complete genomes of Staphylococcus aureus bacteriophages 80 and 80α--implications for the specificity of SaPI mobilization.

Virology·2010

Same author

Climate change and the integrity of science.

Science (New York, N.Y.)·2010

Same author

Antibody markers of incident tuberculosis among HIV-infected adults in the USA: a historical prospective study.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2007

Same author

Serological markers of pulmonary tuberculosis and of response to anti-tuberculosis treatment in a patient population in Guinea.

International journal of immunopathology and pharmacology·2006

Same author

Integration of microscopy and serodiagnostic tests to screen for active tuberculosis.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2005

Area of Science:

Molecular Biology
Microbiology
Genetics

Background:

The plasmid pT181 from Staphylococcus aureus is crucial for understanding bacterial DNA replication mechanisms.
Initiator proteins like RepC play a vital role in the initiation of plasmid replication.
Nucleotide sequence elements can significantly influence the efficiency of DNA replication.

Purpose of the Study:

To investigate the function of the cmp nucleotide sequence element in pT181 plasmid DNA replication.
To determine how cmp affects the competition for the RepC initiator protein.
To characterize the role of cmp in regulating plasmid replication frequency.

Main Methods:

Deletion mutagenesis to remove the cmp sequence from pT181.
Assays to measure plasmid replication frequency under derepressed conditions.
Analysis of competition for the RepC initiator protein between plasmids.

Main Results:

The cmp sequence, located within a 156-base-pair segment, acts as an enhancer of DNA replication.
Presence of cmp on an unrelated vector boosts the pT181 origin's ability to compete for RepC.
Deletion of cmp reduced pT181 replication frequency twofold under derepressed conditions.
The cmp enhancer contains a DNA bending locus, with activity diminishing as distance from the origin increases.

Conclusions:

The cmp sequence is a functional enhancer element that positively regulates pT181 plasmid replication in Staphylococcus aureus.
cmp enhances replication by increasing the efficiency of origin binding or interaction with the RepC initiator protein.
Understanding enhancer elements like cmp provides insights into the regulation of plasmid replication and DNA-protein interactions.

Related Experiment Videos