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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Proteomics01:33

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Related Experiment Video

Updated: Dec 31, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Dissecting intercellular signaling with mass spectrometry-based proteomics.

Jonathan J Swietlik1, Ankit Sinha2, Felix Meissner1

  • 1Experimental Systems Immunology Laboratory, Max-Planck Institute of Biochemistry, Martinsried, Germany.

Current Opinion in Cell Biology
|January 14, 2020
PubMed
Summary

Mass spectrometry-based proteomics offers new ways to study how cells communicate using proteins. This technology helps identify cell signaling pathways involved in diseases.

Keywords:
AutocrineCell non-autonomousHeterocellularInteractionIntercellularLigandMass spectrometryMessenger, InteractionsPTMParacrinePosttranslational modificationProteomicsReceiverReceptorSenderSignaling

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Area of Science:

  • Proteomics
  • Cell Biology
  • Systems Biology

Background:

  • Cellular communication is vital for physiological functions.
  • Investigating intercellular protein signaling in vivo is challenging.
  • Mass spectrometry (MS)-based shotgun proteomics is a powerful emerging technology.

Purpose of the Study:

  • To review advancements in MS-based proteomics for analyzing intercellular protein signaling.
  • To highlight methods for profiling cellular messengers, receptors, and their interactions.
  • To discuss approaches for dissecting cell-specific communication in complex systems.

Main Methods:

  • Cell biological, chemical, and biochemical MS-based approaches.
  • Profiling of released cellular messengers.
  • Analysis of cell surface receptors and their interactions.
  • Mapping dynamic signal transduction at cellular interfaces.
  • Dissecting cell-specific communication in heterocellular systems.

Main Results:

  • MS-based proteomics enables systematic analysis of protein-mediated intercellular signaling.
  • Methods are tailored for profiling released messengers, surface receptors, and interactions.
  • Dynamic signaling mechanisms at cellular interfaces can be mapped.
  • Cell-specific communication in heterocellular systems can be dissected.

Conclusions:

  • MS-based proteomics provides a systems biology perspective on intercellular signaling.
  • This approach is crucial for identifying deregulated signaling pathways in disease.
  • Advancements facilitate a comprehensive understanding of cell-to-cell communication.