Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cation-Directed Host-Guest Assembly and Reversible Wheel to Cluster Interconversion in a 20-Molybdate System.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Oxytocin facilitates social behavior of female rats via selective modulation of interneurons in the medial prefrontal cortex.

Nature communications·2026
Same author

Corrigendum to "Radiological impact of the Fessenheim Nuclear Power Plant decommissioning through prospective Life-Cycle and Risk Assessment approaches" [Science of The Total Environment, Volume 974, 2025, 179200].

The Science of the total environment·2025
Same author

Theoretical design and practical implementation of the food sampling for the 3<sup>rd</sup> French total diet study.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2025
Same author

Separation of acetylcholinesterase inhibitors using high-temperature liquid chromatography: A method development approach.

Journal of chromatography. A·2025
Same author

Novel colorimetric sensor based on aggregation of noble metal nanomaterials for authenticity identification of Lycium ruthenicum from different regions.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2025

Related Experiment Video

Updated: Dec 31, 2025

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory
09:48

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory

Published on: November 7, 2018

14.9K

Deep eutectic solvent-based headspace single-drop microextraction for the quantification of terpenes in spices.

Zélie Triaux1,2, Hugues Petitjean2, Eric Marchioni1

  • 1Université de Strasbourg, CNRS, IPHC UMR 7178, F-67000, Strasbourg, France.

Analytical and Bioanalytical Chemistry
|January 14, 2020
PubMed
Summary

Deep eutectic solvents (DESs) offer a greener approach for extracting terpenes from spices using headspace single-drop microextraction (HS-SDME). This method proved efficient and cost-effective for analyzing bioactive compounds in plants.

Keywords:
ChemometricDeep eutectic solventsGC-MSMicroextractionTerpenes

More Related Videos

Author Spotlight: Exploring Tea Aroma Using Solvent-Assisted Flavor Evaporation Technique
04:36

Author Spotlight: Exploring Tea Aroma Using Solvent-Assisted Flavor Evaporation Technique

Published on: May 26, 2023

4.1K
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

4.4K

Related Experiment Videos

Last Updated: Dec 31, 2025

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory
09:48

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory

Published on: November 7, 2018

14.9K
Author Spotlight: Exploring Tea Aroma Using Solvent-Assisted Flavor Evaporation Technique
04:36

Author Spotlight: Exploring Tea Aroma Using Solvent-Assisted Flavor Evaporation Technique

Published on: May 26, 2023

4.1K
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

4.4K

Area of Science:

  • Analytical Chemistry
  • Green Chemistry
  • Natural Product Chemistry

Background:

  • Traditional methods for extracting volatile compounds like terpenes from spices are often time-consuming and employ harsh solvents.
  • Deep eutectic solvents (DESs) have emerged as promising alternatives due to their unique properties, including low toxicity and tuneability.
  • Headspace single-drop microextraction (HS-SDME) is a solvent-efficient technique for analyzing volatile and semi-volatile compounds.

Purpose of the Study:

  • To investigate the efficacy of various DESs as extraction solvents for HS-SDME.
  • To optimize the HS-SDME method for terpene extraction from selected spices.
  • To evaluate the potential of DES-based HS-SDME for the analysis of bioactive compounds in plant materials.

Main Methods:

  • Screening of 10 DESs, primarily composed of tetrabutylammonium bromide (N4444Br) and long-chain alcohols, for terpene extraction from six spices.
  • Optimization of HS-SDME parameters (extraction time, temperature) using design of experiments and response surface methodology.
  • Analysis of extracted terpenes using gas chromatography coupled to mass spectrometry (GC-MS).
  • Quantitative analysis of identified terpenes using 29 standards.

Main Results:

  • A DES composed of N4444Br and dodecanol (1:2 molar ratio) exhibited the highest extraction efficiency.
  • Optimal extraction conditions were determined to be specific extraction times and temperatures.
  • Over 40 terpenes were identified in nutmeg, with quantitative analysis showing good linearity (R² > 0.99) and low limits of quantification (many below 2 μg/g).

Conclusions:

  • DESs are effective and "greener" solvents for HS-SDME of terpenes from spices.
  • The optimized HS-SDME method using DESs is simple, rapid, and cost-effective compared to conventional techniques.
  • This approach demonstrates suitability for extracting and analyzing bioactive compounds from plant matrices.