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LncRNA SBF2-AS1 affects the radiosensitivity of non-small cell lung cancer via modulating microRNA-302a/MBNL3 axis

Zhanwu Yu1, Gebang Wang1, Chenlei Zhang1

  • 1Department of Thoracic Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, Liaoning, P.R. China.

Cell Cycle (Georgetown, Tex.)
|January 14, 2020
PubMed

Insights

Inhibiting lncRNA SBF2-AS1 or increasing microRNA-302a enhances non-small cell lung cancer radiosensitivity. This modulation targets MBNL3, offering a potential therapeutic strategy for NSCLC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in non-small cell lung cancer (NSCLC) pathogenesis.
  • Understanding lncRNA roles is crucial for developing novel NSCLC therapies.

Purpose of the Study:

  • To investigate the effect of lncRNA SBF2-AS1 on microRNA-302a (miR-302a) expression.
  • To determine the impact of this interaction on NSCLC radiosensitivity.

Main Methods:

  • Assessed SBF2-AS1, miR-302a, and MBNL3 expression in NSCLC tissues and cell lines.
  • Utilized cell transfection with si-SBF2-AS1 and miR-302a mimics.
  • Evaluated proliferation, apoptosis, and radiosensitivity in vitro and tumor growth in vivo.

Main Results:

  • Radiotherapy-resistant NSCLC tissues and cells showed elevated SBF2-AS1 and MBNL3, with decreased miR-302a.
  • Downregulating SBF2-AS1 or upregulating miR-302a reduced proliferation and increased apoptosis in NSCLC cells.
  • Inhibition of SBF2-AS1 or enhancement of miR-302a decreased radioresistance and suppressed tumor growth in vivo.

Conclusions:

  • High miR-302a expression or SBF2-AS1 inhibition enhances NSCLC radiosensitivity and apoptosis.
  • This effect is mediated through the downregulation of MBNL3.
  • MBNL3 represents a potential therapeutic target for improving NSCLC radiosensitivity.
Keywords:
ApoptosisLncRNA SBF2-AS1MicroRNA-302aMuscleblind-like 3Non-small cell lung cancerProliferationRadiosensitivity

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