Related Experiment Video

Updated: Dec 31, 2025

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
08:17

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Published on: October 30, 2016

21.8K

The Monoclonal Antibody NEO-201 Enhances Natural Killer Cell Cytotoxicity Against Tumor Cells Through Blockade of the

Massimo Fantini1, Justin M David1, Christina M Annunziata2

  • 1Precision Biologics, Inc., Bethesda, Maryland.

Cancer Biotherapy & Radiopharmaceuticals
|January 14, 2020
PubMed

Insights

NEO-201 antibody blocks the interaction between CEACAM5 on tumor cells and CEACAM1 on natural killer (NK) cells. This blockade restores NK cell

Area of Science:

  • Immunology
  • Oncology
  • Cell Biology

Background:

  • Natural killer (NK) cells are crucial for innate immunity and cancer surveillance.
  • The interaction between carcinoembryonic antigen (CEA) on tumor cells and CEACAM1 on NK cells inhibits NK cell anti-tumor activity.
  • CEACAM1 is a key regulator of NK cell function.

Purpose of the Study:

  • To investigate if NEO-201 can block the CEACAM5/CEACAM1 interaction.
  • To determine if blocking this interaction restores NK cell-mediated anti-tumor cytotoxicity.
  • To assess the efficacy of NEO-201 in enhancing NK cell activity against CEACAM5-expressing tumors.

Main Methods:

  • Utilized in vitro functional assays with human tumor cell lines and NK-92 cells.
  • Assessed NEO-201's ability to block CEACAM5 on tumor cells and CEACAM1 on NK cells.
  • Employed NK-92 cells as a model for direct NK cell killing, lacking antibody-dependent cellular cytotoxicity.

Main Results:

  • Expression profiling showed varied CEACAM5 and NEO-201 expression on carcinoma cell lines.
  • NEO-201 significantly enhanced NK-92 cell cytotoxicity against CEACAM5+/NEO-201+ tumor cells.
  • The enhanced cytotoxicity correlated with the level of CEACAM5+/NEO-201+ expression.

Conclusions:

  • NEO-201 effectively blocks the CEACAM5-CEACAM1 interaction.
  • NEO-201 reverses CEACAM1-dependent inhibition of NK cell cytotoxicity.
  • NEO-201 holds potential for enhancing anti-tumor immunity in CEACAM5-expressing cancers.
Keywords:
CEACAM5NEO-201NK cell cytotoxicityimmune checkpoint pathwaymonoclonal antibodynatural killer cells

More Related Videos

Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies09:54

Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies

Published on: May 22, 2020

3.8K
Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells11:08

Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells

Published on: February 14, 2025

1.7K

Related Concept Videos

Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
1.7K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
6.3K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
7.7K
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
8.4K