Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

10.3K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
10.3K
Enzyme Inhibition01:30

Enzyme Inhibition

90.7K
Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
90.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ligand Radicals Tune LPMO Activity in Model Complex.

Inorganic chemistry·2026
Same author

Asymmetrically glycosylated IgG1 antibodies are universal and drive human disease.

Nature communications·2025
Same author

FcγRI is the key determinant of antibody-mediated Zika virus infection of human placental macrophages.

bioRxiv : the preprint server for biology·2025
Same author

Ascorbyl Radicals as Reducing Agents in Copper-Catalyzed Redox Reactions.

Inorganic chemistry·2025
Same author

Molecular basis of Fab-dependent IgA antibody recognition by gut-bacterial metallopeptidases.

The EMBO journal·2025
Same author

The mechanistic basis for interprotomer deglycosylation of antibodies by corynebacterial IgG-specific endoglycosidases.

Nature communications·2025

Related Experiment Video

Updated: Dec 31, 2025

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
11:16

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Published on: July 11, 2012

16.7K

Strategies to rationalize enzyme immobilization procedures.

Diego E Sastre1, Eduardo A Reis1, Caterina G C Marques Netto1

  • 1Laboratório de Metaloenzimas e Biomiméticos, Departamento de Química, Universidade Federal de São Carlos (UFSCar), São Paulo, Brazil.

Methods in Enzymology
|January 15, 2020
PubMed
Summary
This summary is machine-generated.

Rational enzyme immobilization uses bioinformatics and data analysis to reduce empirical methods. Strategies like pH modulation and protein engineering enhance enzyme stability and reusability for industrial applications.

Keywords:
EmpiricismEnzyme immobilizationEnzyme immobilization in silico predictionFactorial planningGlutaraldehyde cross-linkingIron-sensitive enzymesRationalization of protocolsSite-specific immobilization

More Related Videos

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

18.0K
The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

21.0K

Related Experiment Videos

Last Updated: Dec 31, 2025

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
11:16

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Published on: July 11, 2012

16.7K
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

18.0K
The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

21.0K

Area of Science:

  • Biotechnology
  • Biochemistry
  • Enzyme Engineering

Background:

  • Enzyme immobilization is crucial for enzyme stability, activity, and reusability.
  • Current immobilization methods often rely on empirical approaches, leading to inefficiencies.
  • Reducing empiricism in enzyme immobilization is key for optimizing biocatalytic processes.

Purpose of the Study:

  • To present rational design strategies for enzyme immobilization protocols.
  • To decrease the reliance on empirical methods in enzyme immobilization.
  • To enhance the operational stability and integrity of immobilized enzymes.

Main Methods:

  • Modulation of immobilization pH for glutaraldehyde cross-linked enzymes.
  • Coating iron-containing supports for iron-sensitive enzymes.
  • Application of factorial planning, protein engineering, and in silico tools.
  • Development of a public repository for rational design data.

Main Results:

  • Rational design approaches can significantly reduce empiricism in enzyme immobilization.
  • Specific strategies effectively regulate operational stability and preserve enzyme integrity.
  • In silico tools and data repositories offer promising avenues for future improvements.

Conclusions:

  • Rational design principles are essential for advancing enzyme immobilization technology.
  • Optimized immobilization protocols lead to more stable, active, and reusable enzymes.
  • Collaborative data sharing through repositories can accelerate innovation in enzyme immobilization.