Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tyrosinase Cross-Linked PEG Hydrogels with DAT and DATT as Artificial Substrates: Design, Structure, and Functions.

Biomacromolecules·2026
Same author

Substrates mimicking the blastocyst geometry revert pluripotent stem cell to naivety.

Nature materials·2024
Same author

Multivariate Analysis of Cellular Uptake Characteristics for a (Co)polymer Particle Library.

ACS biomaterials science & engineering·2024
Same author

Opportunities and challenges for integrating the development of sustainable polymer materials within an international circular (bio)economy concept.

MRS energy & sustainability : a review journal·2023
Same author

Rapid depolymerization of poly(ethylene terephthalate) thin films by a dual-enzyme system and its impact on material properties.

Chem catalysis·2023
Same author

Histone Modification of Osteogenesis Related Genes Triggered by Substrate Topography Promotes Human Mesenchymal Stem Cell Differentiation.

ACS applied materials & interfaces·2023

Related Experiment Video

Updated: Dec 31, 2025

Fabrication of Carbon-Based Ionic Electromechanically Active Soft Actuators
14:42

Fabrication of Carbon-Based Ionic Electromechanically Active Soft Actuators

Published on: April 25, 2020

8.7K

Polymeric sheet actuators with programmable bioinstructivity.

Zijun Deng1,2,3, Weiwei Wang1,2, Xun Xu1,2

  • 1Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, 14513 Teltow, Germany.

Proceedings of the National Academy of Sciences of the United States of America
|January 15, 2020
PubMed
Summary
This summary is machine-generated.

Researchers synchronized thermal and mechanical signals for mesenchymal stem cells (MSCs) using a programmable polymer sheet. This accelerated osteogenic differentiation by linking temperature and mechanical sensing via calcium signaling, impacting bone defect tissue engineering.

Keywords:
HDAC1RUNX2calcium influxmesenchymal stem cellsreversible shape-memory actuator

More Related Videos

Bioinspired Soft Robot with Incorporated Microelectrodes
08:24

Bioinspired Soft Robot with Incorporated Microelectrodes

Published on: February 28, 2020

9.2K
Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot - PART 1
11:22

Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot - PART 1

Published on: July 11, 2017

8.4K

Related Experiment Videos

Last Updated: Dec 31, 2025

Fabrication of Carbon-Based Ionic Electromechanically Active Soft Actuators
14:42

Fabrication of Carbon-Based Ionic Electromechanically Active Soft Actuators

Published on: April 25, 2020

8.7K
Bioinspired Soft Robot with Incorporated Microelectrodes
08:24

Bioinspired Soft Robot with Incorporated Microelectrodes

Published on: February 28, 2020

9.2K
Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot - PART 1
11:22

Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot - PART 1

Published on: July 11, 2017

8.4K

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Mesenchymal stem cells (MSCs) respond to environmental cues like mechanical, thermal, and biochemical signals to differentiate.
  • Typically, these stimuli are applied independently, necessitating complex control systems.
  • Understanding the interplay of these signals is crucial for advanced tissue engineering applications.

Purpose of the Study:

  • To develop a method for autonomously synchronizing thermal and mechanical signals applied to MSCs.
  • To investigate the interconnectedness of temperature and mechanosensing in MSCs.
  • To leverage this interplay to accelerate osteogenic differentiation for bone tissue regeneration.

Main Methods:

  • Utilized a programmable polymer actuator sheet to deliver synchronized thermal and mechanical stimuli.
  • Employed an integrated grid system for real-time visualization and quantification of cell morphology, calcium influx, and focal adhesion assembly.
  • Analyzed changes in histone H3K9 acetylation and osteogenic gene expression.

Main Results:

  • Demonstrated that temperature and mechanosensing in MSCs are interconnected through intracellular calcium (Ca2+) influx.
  • Showed that up-regulated Ca2+ levels significantly alter histone acetylation and activate osteogenic genes.
  • Successfully accelerated MSC differentiation towards an osteogenic lineage by combining physical, thermal, and biochemical signaling.

Conclusions:

  • The programmable bioinstructivity approach offers a fundamental principle for designing functional biomaterials that utilize multifaceted stimuli for cell differentiation.
  • The synchronized application of thermal and mechanical signals via programmable actuators enhances osteogenic differentiation.
  • This technology holds significant potential for periosteum tissue engineering to treat bone defects.