Related Experiment Video

Updated: Dec 31, 2025

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

16.0K

Author Correction: Split intein-mediated selection of cells containing two plasmids using a single antibiotic

Navaneethan Palanisamy1,2,3, Anna Degen2,4,5, Anna Morath1,6,7,8

  • 1Signalling Research Centres BIOSS and CIBSS, University of Freiburg, 79104, Freiburg, Germany.

Nature Communications
|January 15, 2020
PubMed

Abstract:

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

More Related Videos

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

16.9K
Use of In Vivo Assembly for High-efficiency Plasmid Construction
06:25

Use of In Vivo Assembly for High-efficiency Plasmid Construction

Published on: February 7, 2025

1.3K

Related Experiment Videos

Last Updated: Dec 31, 2025

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

16.0K
Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

16.9K
Use of In Vivo Assembly for High-efficiency Plasmid Construction
06:25

Use of In Vivo Assembly for High-efficiency Plasmid Construction

Published on: February 7, 2025

1.3K

Related Concept Videos

Antibiotic Selection00:57

Antibiotic Selection

59.2K
Overview
59.2K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.5K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.5K

Articles linked to this work by shared authors, journal, and citation graph.

Vitamin C enhances cytotoxicity of CAR-and εTRuC-engineered γδ T cells and modulates Ca2+, NFAT, NF-κB and ERK signaling in human γδ T cells.

Frontiers in immunology·2026

The Y105 phosphosite between the SH3.1 and SH3.2 domains of Nck1 regulates its binding to CD3ϵ.

iScience·2026

Distinct postnatal trajectories of mouse dendritic epidermal T cells and Langerhans cells independent of microbiota.

Science advances·2026

USP8 controls proteostasis pathways in B cells and multiple myeloma.

Leukemia·2026

Nutrition knowledge, attitudes toward physical activity and malnutrition as predictors of social appearance anxiety: a structural equation modeling approach.

Frontiers in nutrition·2025

Opto-CD28-REACT: optogenetic co-stimulatory receptor activation on non-engineered human T cells.

Frontiers in immunology·2025

DeepIR-Pred: identification of insulin receptors using metaheuristic optimization of biologically informed multi-view features with deep recurrent learning.

Briefings in bioinformatics·2026

Deubiquitinase USP8 inhibits HIV-1 replication by suppressing Tat-mediated viral transactivation.

Journal of virology·2026

Site-specific inhibition of translation initiation via 2'-O-methylation.

Nucleic acids research·2026

A Dual-Plasmid CRISPR/Cas9-Based Genome Editing System for Efficient Gene Knockout and Large DNA Integration in Cronobacter malonaticus.

Pathogens (Basel, Switzerland)·2026

A Biological Approach to Antimicrobial Resistance in New and Old Bacterial Pathogens.

Pathogens (Basel, Switzerland)·2026

Diverse Horizontally Transferred Cellulose Biosynthesis Gene Clusters in Escherichia coli Strains.

Microorganisms·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us