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Emphysema in the Blotchy mouse: a morphometric study.

A C McCartney1, B Fox, T A Partridge

  • 1Department of Histopathology, Charing Cross and Westminster Medical School, London, U.K.

The Journal of Pathology
|September 1, 1988
PubMed
Summary
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Researchers developed a new method to detect emphysema in mice, identifying the condition in heterozygous Blotchy mice. This technique offers a sensitive and accurate way to analyze lung changes associated with the Blotchy allele.

Area of Science:

  • Pulmonary Pathology
  • Genetics
  • Medical Imaging Analysis

Background:

  • The Blotchy (Blo) allele on the X-chromosomal Mottled locus in mice causes lung alterations resembling emphysema.
  • Previous methods failed to detect emphysema in heterozygous Blo/+ females, despite its presence in hemizygous Blo/Y males and homozygous Blo/Blo females.

Purpose of the Study:

  • To develop a rapid and accurate method for analyzing emphysema in mouse models.
  • To determine if the new method could identify emphysema in heterozygous Blo/+ females.

Main Methods:

  • Mouse lungs (wild type and Blo strains) were inflated and fixed.
  • Computerized image analysis measured transect lengths across air spaces.
  • Statistical analysis included Kolmogorov-Smirnov tests on cumulative frequency data.

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Main Results:

  • Significantly increased transect lengths, indicative of emphysema, were observed in Blo/Blo females and Blo/Y males compared to wild-type controls (P < 0.001).
  • Heterozygous Blo/+ females exhibited intermediate transect lengths, significantly different from both wild-type and Blotchy mice (P < 0.001).
  • The method demonstrated sensitivity by distinguishing Blo/+ females as an intermediate group.

Conclusions:

  • A novel computerized image analysis method accurately quantifies emphysema in mouse lungs.
  • This method successfully identified emphysema in heterozygous Blo/+ females, previously undetected.
  • The findings highlight the utility of this technique for studying genetic models of lung disease.