Exploration of T-Cell Diversity Using Mass Cytometry

Area of Science:

• Immunology
• Biotechnology
• Computational Biology

Background:

• T-cell diversity encompasses antigen specificity, differentiation, function, and trafficking.
• Traditional flow cytometry has limitations in capturing T-cell subset diversity due to spectral overlap.
• Mass cytometry offers deep immunoprofiling capabilities for T-cell subsets, activation, and function.

Purpose of the Study:

• To describe methods for mass cytometry and high-throughput data analysis of T cells in patient cohorts.
• To provide a practical pipeline for customizing mass cytometry panels.
• To support translational science through detailed workflows for high-dimensional data evaluation.

Main Methods:

• Mass cytometry for deep immunoprofiling of T-cell subsets.
• High-throughput data analysis pipelines for patient cohorts.
• Protocols for metal-labeled antibody conjugation, titration, and staining procedures.

Main Results:

• Development of a mass cytometry pipeline for T-cell analysis.
• Detailed protocols for antibody conjugation and staining.
• Provision of R scripts for high-dimensional data evaluation.

Conclusions:

• Mass cytometry significantly enhances the ability to study T-cell diversity and function.
• The provided methods and scripts facilitate translational research in immunology.
• This work offers a comprehensive approach to T-cell immunoprofiling in patient cohorts.