Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MARBL: A Live-Cell Method for Profiling Bioenergetic Heterogeneity by Noncanonical Methionine Labeling of the Cell Surface Proteome.

bioRxiv : the preprint server for biology·2026
Same author

A combined radiomics and habitat analysis model for predicting early recurrence of HCC after liver transplantation.

Frontiers in oncology·2026
Same author

Sodium butyrate stimulates intestinal health and immunity by reshaping the intestinal microbiota and metabolite profile in big-belly seahorses (Hippocampus abdominalis).

Comparative biochemistry and physiology. Part D, Genomics & proteomics·2026
Same author

T-cell protrusions enable fast, localised initiation of chimeric antigen receptor signalling.

The EMBO journal·2026
Same author

Sirtuin 7-mediated deacetylation of hypoxia-inducible factor 1-alpha facilitates glycolytic reprogramming and inflammation of keratinocytes in psoriasis.

Journal of advanced research·2026
Same author

Mast cell extracellular granules are bioactive condensates assembled by heparin and polyamine.

Nature chemical biology·2026

Related Experiment Video

Updated: Dec 31, 2025

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography
09:34

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography

Published on: February 17, 2022

3.7K

Imaging Chimeric Antigen Receptor (CAR) Activation.

Kendra A Libby1, Xiaolei Su2

  • 1Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 15, 2020
PubMed
Summary

Chimeric antigen receptor (CAR) T cell activation mechanisms are unclear. This study introduces a microscopy method using supported lipid bilayers and TIRF microscopy to visualize CAR signaling dynamics at high resolution.

Keywords:
CD19Chimeric antigen receptorMicroclustersT cell signalingTIRF

More Related Videos

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
11:12

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity

Published on: April 11, 2019

7.4K
A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
08:46

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

Published on: November 12, 2019

53.9K

Related Experiment Videos

Last Updated: Dec 31, 2025

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography
09:34

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography

Published on: February 17, 2022

3.7K
Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
11:12

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity

Published on: April 11, 2019

7.4K
A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
08:46

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

Published on: November 12, 2019

53.9K

Area of Science:

  • Immunology
  • Cell Biology
  • Biophysics

Background:

  • Chimeric antigen receptor (CAR) T cell therapy is a promising cancer immunotherapy.
  • The precise molecular mechanisms of CAR T cell activation by tumor antigens remain poorly understood.
  • Understanding CAR signaling is crucial for improving CAR T cell efficacy.

Purpose of the Study:

  • To investigate the molecular mechanisms of CAR T cell activation.
  • To develop a high-resolution method for visualizing CAR signaling.
  • To address key questions regarding CAR activation and signal transduction.

Main Methods:

  • Development of a microscopy-based assay for CAR signaling studies.
  • Utilizing antigen-coated supported lipid bilayers to trigger CAR activation.
  • Employing Total Internal Reflection Fluorescence (TIRF) microscopy to visualize early CAR T cell activation events.

Main Results:

  • The study presents a novel method for observing CAR signaling.
  • Visualized initial CAR activation processes at the cell surface.
  • Achieved high spatial and temporal resolution in monitoring CAR signaling dynamics.

Conclusions:

  • The developed microscopy technique provides unprecedented insight into CAR signaling.
  • This method facilitates the study of fundamental CAR T cell activation processes.
  • Further research using this technique can optimize CAR T cell-based cancer immunotherapies.