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A plasmonic thermal sensing based portable device for lateral flow assay detection and quantification.

Zhuo Qu1, Kan Wang1, Gabriel Alfranca2,3

  • 1Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent diagnosis and treatment instrument, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai, 200240, China.

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Summary

This study enhances lateral flow assays (LFAs) for point-of-care testing (POCT) by enabling quantification. Optimized thermal sensing modes significantly improve detection limits for early disease diagnosis.

Keywords:
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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Point-of-care testing (POCT) is crucial for disease diagnosis and monitoring.
  • Lateral flow assays (LFAs) are common POCT tools but lack quantification and sensitivity.
  • Previous thermal LFAs improved sensitivity but not quantification.

Purpose of the Study:

  • To optimize thermal sensing modes for quantitative LFAs.
  • To improve the analytical sensitivity and quantification capabilities of thermal LFAs.
  • To validate the thermal sensing method for biomarker detection.

Main Methods:

  • Developed a portable device for thermal LFAs using plasmonic gold nanoparticles.
  • Optimized conduction and radiation thermal sensing modes.
  • Studied the effect of ambient temperature on detection modes.
  • Quantified human chorionic gonadotropin (HCG) using the optimized LFA.

Main Results:

  • Detection limits were reduced 5-fold (conduction) and 12-fold (radiation) compared to visual detection.
  • Radiation mode was more sensitive to ambient temperature variations than conduction mode.
  • Achieved a detection limit of 2.8 mIU/mL for HCG using the radiation method.

Conclusions:

  • Optimized thermal sensing modes enable quantification in LFAs.
  • The developed thermal LFA system significantly enhances analytical sensitivity and detection limits.
  • This method offers a promising approach for sensitive and quantitative POCT.