Spontaneous flight activity of Aedes trivittatus infected with Dirofilaria immitis

Spontaneous flight activity of Dirofilaria immitis-infected Aedes trivittatus was evaluated by using an acoustic activity system. The activity of mosquitoes infected with low numbers of filarial larvae (1-4) was similar to that of uninfected mosquitoes. However, mosquitoes infected with more than 4 larvae became more active than uninfected mosquitoes 8 days after infection. Peak flight activity (circadian) occurred at the same time in both infected and uninfected mosquitoes, but infected mosquitoes were much more active during normal periods of quiescence. Flight activity of mosquitoes infected with more than 4 larvae was suppressed on days 10 and 14 postinfection, corresponding to times of greatest disruption of the Malpighian tubules by the developing larvae.