Expression of CD44 and MDM2 in cholangiocarcinoma is correlated with poor clinicopathologic characteristics

The aim of the study was to investigate the expression of cluster of differentiation 44 (CD44) and mouse double minute 2 (MDM2) in cholangiocarcinoma, in addition to evaluating their association with clinicopathologic characteristics and overall survival time. Paraffin-embedded tumor tissues from 128 patients from 3 study centers in Thailand were evaluated using immunohistochemistry. The results demonstrated that positive expression of CD44 was associated with high histologic grade (P=0.013), large tumor size (P=0.027), lymph node metastasis (P=0.037), and distant metastasis (P=0.031). MDM2 expression was related to high histologic grade (P=0.013), lymph node metastasis (P=0.025), and distant metastasis (P=0.016). Furthermore, multivariate analyses revealed that combined expression of CD44 and MDM2 was significantly associated with worse overall survival time (OR=1.52; 95% CI=1.04-2.26; P=0.041) in patients with cholangiocarcinoma. CD44 and MDM2 significantly indicate poor clinicopathologic outcomes in patients with cholangiocarcinoma.