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Updated: Dec 31, 2025

MicroRNA-based Regulation of Picornavirus Tropism
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MicroRNA-221 inhibits human papillomavirus 16 E1-E2 mediated DNA replication through activating SOCS1/Type I IFN

Haikong Lu1, Xin Gu1

  • 1Department of Sexually Transmitted Disease Institute, Shanghai Skin Disease Hospital Shanghai 200050, China.

International Journal of Clinical and Experimental Pathology
|January 15, 2020
PubMed

Insights

MicroRNA-221 (miR-221) inhibits Human Papillomavirus (HPV) 16 replication by targeting SOCS1 and modulating the Type I Interferon (IFN) immune response. This discovery suggests miR-221 as a potential therapeutic target for HPV infections.

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Human Papillomavirus (HPV) 16 is a major cause of cervical cancer.
  • MicroRNAs (miRNAs) are implicated in viral infections and immune responses.
  • The role of miRNAs in HPV 16 infection requires further elucidation.

Purpose of the Study:

  • To identify and evaluate miRNAs involved in HPV 16 replication.
  • To understand the mechanism of miRNA regulation of the Type I Interferon (IFN) immune response in HPV 16 infection.

Main Methods:

  • Microarray and quantitative real-time PCR (qRT-PCR) assays were used.
  • In vitro experiments assessed HPV 16 DNA replication upon miR-221 manipulation.
  • Western blotting and qRT-PCR analyzed protein and mRNA expression of IFN-α, IFN-β, and Interferon Stimulated Genes (ISGs).
  • Luciferase reporter assays identified direct targets of miR-221.

Main Results:

  • MicroRNA-221 (miR-221) was significantly upregulated in HPV 16-infected patients and cells.
  • miR-221 overexpression inhibited HPV 16 replication, while knockdown facilitated it.
  • miR-221 upregulated IFN-α, IFN-β, and ISG expression, including MxA, OAS, and ISG15.
  • Suppressor of Cytokine Signaling 1 (SOCS1) was identified as a direct target of miR-221.

Conclusions:

  • miR-221 inhibits HPV 16 replication by targeting SOCS1, thereby modulating the Type I IFN signaling pathway.
  • miR-221 plays a crucial role in the host's immune response to HPV 16 infection.
  • miR-221 represents a potential novel therapeutic target for HPV infections.
Keywords:
DNA replicationHuman papillomavirus 16SOCS1/Type I IFN signaling pathwaymicroRNA-221

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