Related Experiment Video
Updated: Dec 31, 2025

Differentiated Mouse Adipocytes in Primary Culture: A Model of Insulin Resistance
Published on: February 17, 2023
Vitamin D deficiency enhances insulin resistance by promoting inflammation in type 2 diabetes
Weichao Wang1, Jie Zhang2, Hong Wang3
1Department of Endocrinology, Shijiazhuang First Hospital Shijiazhuang 050011, Hebei, China.
Objective:
This study was aimed to analyze the level of serum 25(OH)D in patients with type 2 diabetes (T2DM), and explore the relationship between serum 25(OH)D and insulin resistance.
Methods:
60 health people and 106 patients with T2DM were measured the level of serum 25(OH)D, fasting blood glucose, insulin, TNF-α, IL-6, IL-8, and IL-1β, etc. We established a rat model of T2DM and vitamin D (VD) deficiency, and studied the effects of VD deficiency on homeostasis model assessment insulin resistance (HOMA-IR) and pancreatic inflammation.
Results:
The level of serum 25(OH)D in patients with T2DM was significantly lower than that in health people, and HOMA-IR decreased with the increasing of the serum 25(OH)D level. Pearson correlation analysis showed that the serum 25(OH)D level in patients with T2DM had a negative correlation with HOMA-IR (r=-0.750, P<0.001), TNF-α (r=-0.705, P<0.001), IL-1β (r=-0.661, P<0.001), IL-8 (r=-0.645, P<0.001), and IL-6 (r=-0.609, P<0.001). In animal experiment, Vitamin D deficiency enhanced HOMA-IR in rats with T2DM and reversed it by supplementing VD. Vitamin D deficiency could increase the inflammatory response by up-regulating p-p65/RelB in the pancreas tissue.
Conclusion:
Serum 25(OH)D was elevated and Vitamin D deficiency enhanced insulin resistance by promoting inflammation via NF-kB pathway in patients with T2DM.
More Related Videos
08:47Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
Published on: December 7, 2017
08:13Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test OGTT and Insulin Tolerance Test ITT
Published on: January 7, 2018
Related Concept Videos
Diabetes Mellitus: Type 2 and Gestational
Diabetes Mellitus: Overview and Type I Subtype
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
Carbohydrate Metabolism
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
Pathophysiology of Diabetes
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
Oral Hypoglycemic Agents: Biguanides and Glitazones
Psychoneuroimmunology: Diabetes and Cancer