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MiRNA-134 suppresses esophageal squamous cell carcinoma progression by targeting FOXM1

Yuan Yuan1, Qian Wang2, Fangfang Cao1

  • 1Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University Qingdao 266000, Shandong Province, People's Republic of China.

International Journal of Clinical and Experimental Pathology
|January 15, 2020
PubMed

Insights

MicroRNA-134 (miR-134) inhibits esophageal squamous cell carcinoma (ESCC) cell invasion and migration by targeting FOXM1. This finding suggests miR-134 as a potential therapeutic target for ESCC treatment.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNA (miRNA) dysregulation is implicated in cancer progression.
  • Esophageal squamous cell carcinoma (ESCC) is a significant global health concern.
  • Specific miRNAs, including miR-134, are investigated for their roles in ESCC metastasis.

Purpose of the Study:

  • To investigate the inhibitory effect of miR-134 on ESCC cell invasion and migration.
  • To determine the relationship between miR-134 expression and key molecular targets in ESCC.
  • To explore the potential of miR-134 as a therapeutic target for ESCC.

Main Methods:

  • Quantitative real-time PCR (RT-PCR) to assess miRNA and gene expression in ESCC tissues.
  • Western blot to analyze protein expression of MMP-2, MMP-9, COL1A1, COL1A5, and FOXM1.
  • In vitro assays including wound healing and Transwell chambers to evaluate cell migration and invasion.
  • Luciferase reporter assay and gene overexpression to confirm target interactions.

Main Results:

  • miR-134 expression was significantly decreased in ESCC tissues compared to normal tissues.
  • Expression of MMP-2, MMP-9, COL1A1, COL1A5, and FOXM1 was significantly increased in ESCC tissues.
  • miR-134 mimics reduced ESCC cell migration, invasion, and expression of target genes in vitro.
  • miR-134 directly targeted FOXM1, inhibiting its activity and downstream effects.

Conclusions:

  • miR-134 suppresses ESCC cell migration and invasion by targeting the FOXM1 pathway.
  • Restored miR-134 levels inhibit key proteins involved in extracellular matrix degradation and cell motility.
  • miR-134 represents a promising novel therapeutic target for esophageal squamous cell carcinoma.
Keywords:
Esophageal squamous cell carcinomaforkhead box M1invasionmiR-134migration

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