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LncRNA PCAT-1 plays an oncogenic role in epithelial ovarian cancer by modulating cyclinD1/CDK4 expression

Can Ding1, Ruqiong Wei1, Raquel Alarcón Rodríguez1

  • 1Department of Nursing, Physiotherapy and Medicine, Universidad de Almería Almería, Spain.

International Journal of Clinical and Experimental Pathology
|January 15, 2020
PubMed

Insights

Long non-coding RNA PCAT-1 is upregulated in epithelial ovarian cancer (EOC), promoting tumor growth and progression. Silencing PCAT-1 inhibits EOC cell proliferation and invasion by downregulating cyclin D1 and CDK4, suggesting PCAT-1 as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Epithelial ovarian cancer (EOC) is a leading cause of gynecological cancer mortality.
  • The role of long non-coding RNA PCAT-1 in EOC remains largely uncharacterized.
  • PCAT-1 has demonstrated tumor-suppressive functions in other cancer types.

Purpose of the Study:

  • To investigate the expression and function of LncRNA PCAT-1 in epithelial ovarian cancer.
  • To determine the association between PCAT-1 expression and clinicopathological features of EOC.
  • To elucidate the underlying molecular mechanisms of PCAT-1's role in EOC progression.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for gene expression analysis.
  • In vitro assays including cell proliferation, migration, invasion, and cell cycle analysis.
  • Western blot analysis to assess protein expression levels of cyclin D1 and CDK4.

Main Results:

  • LncRNA PCAT-1 expression is significantly upregulated in EOC tissues compared to non-cancerous controls.
  • Higher PCAT-1 expression correlates with larger tumor size and advanced tumor grade in EOC patients.
  • Silencing PCAT-1 in EOC cell lines (SKOV3, OVCAR3) inhibited cell proliferation, migration, and invasion, and increased G0/G1 phase arrest.
  • PCAT-1 knockdown led to decreased protein expression of cyclin D1 and CDK4.

Conclusions:

  • LncRNA PCAT-1 acts as an oncogene in epithelial ovarian cancer.
  • PCAT-1 promotes EOC progression by mediating the cyclin D1/CDK4 pathway.
  • Targeting LncRNA PCAT-1 presents a potential therapeutic strategy for EOC treatment.
Keywords:
LncRNA PCAT-1cyclinD1/CDK4epithelial ovarian cancer

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