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Related Experiment Video

Updated: Dec 31, 2025

Super-Resolution Live Cell Imaging of Subcellular Structures
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A Trojan Horse for live-cell super-resolution microscopy.

Gerti Beliu1, Markus Sauer1

  • 1Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany.

Light, Science & Applications
|January 15, 2020
PubMed
Summary

New peptide vehicles allow easy live-cell labeling of intracellular organelles using fluorescent probes. This method enhances multicolor super-resolution fluorescence imaging capabilities.

Keywords:
Applied opticsOptical techniques

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Microscopy

Background:

  • Live-cell labeling of intracellular organelles is crucial for understanding cellular functions.
  • Existing methods often require complex procedures or are limited in probe accessibility.
  • Cell-impermeable fluorescent probes are valuable tools but struggle to enter live cells efficiently.

Discussion:

  • Developed novel peptide vehicles for efficient delivery of cell-impermeable fluorescent probes into live cells.
  • Demonstrated simple coincubation protocol for organelle labeling, bypassing complex cell permeabilization steps.
  • Enabled precise and efficient labeling of various intracellular organelles.

Key Insights:

  • Peptide vehicles facilitate the translocation of cell-impermeable probes across the plasma membrane.
  • Achieved high-quality live-cell labeling, preserving cell viability and organelle integrity.
  • Successfully applied the method for multicolor super-resolution fluorescence imaging.

Outlook:

  • Potential for broader applications in studying dynamic cellular processes in real-time.
  • Facilitates advancements in high-resolution live-cell imaging techniques.
  • Opens new avenues for drug delivery and diagnostic imaging research.