Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Composition-tunable hyaluronic acid/zwitterionic hybrid fibrous biointerfaces for programmable CD44-mediated cell adhesion and flow-triggered release.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Effect of ranibizumab on diabetic retinopathy <i>via</i> the vascular endothelial growth factor/STAT3/glial fibrillary acidic protein pathway.

World journal of diabetes·2025
Same author

Clipping noise-free transmission scheme in OFDM-based visible light communications.

Optics letters·2025
Same author

The proliferation and differentiation of skeletal muscle stem cells are enhanced in a bioreactor.

Biotechnology and bioengineering·2024
Same author

Development of a multifunctional bioreactor to evaluate the promotion effects of cyclic stretching and electrical stimulation on muscle differentiation.

Bioengineering & translational medicine·2024
Same author

Potential prognostic and predictive value of UBE2N, IMPDH1, DYNC1LI1 and HRASLS2 in colorectal cancer stool specimens.

Biomedical reports·2023
Same journal

RETRACTED: Alshabanah et al. Elastic Nanofibrous Membranes for Medical and Personal Protection Applications: Manufacturing, Anti-COVID-19, and Anti-Colistin Resistant Bacteria Evaluation. <i>Polymers</i> 2021, <i>13</i>, 3987.

Polymers·2026
Same journal

Correction: Kang et al. Energy-Saving Electrospinning with a Concentric Teflon-Core Rod Spinneret to Create Medicated Nanofibers. <i>Polymers</i> 2020, <i>12</i>, 2421.

Polymers·2026
Same journal

Influence of Self-Adhesive Resin Composite Deep Marginal Elevation on the Sealing Ability of CAD/CAM Lithium Disilicate Glass-Ceramic Inlays: An In Vitro Study.

Polymers·2026
Same journal

Modulating Exciton Dynamics Through Fluorescent Side Group Incorporation in Benzodithiophene-Benzotriazole-Isoindigo Terpolymers.

Polymers·2026
Same journal

PLA/PBSA Biocomposites Reinforced with Tangerine Tree-Derived Agro-Industrial Waste for Rigid Packaging: Effect of Extraction Treatment on Morphology and Thermo-Mechanical Performance.

Polymers·2026
Same journal

Synergistic Coatings Based on Chitosan and <i>Eugenia caryophyllata</i> Essential Oil to Improve Postharvest Quality of <i>Capsicum chinense</i>.

Polymers·2026
See all related articles

Related Experiment Video

Updated: Dec 31, 2025

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
04:46

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

Published on: September 12, 2011

10.7K

Electrical Field-Assisted Gene Delivery from Polyelectrolyte Multilayers.

Yu-Che Cheng1,2,3, Shu-Lin Guo3,4,5, Kun-Da Chung6

  • 1Proteomics Laboratory, Department of Medical Research, Cathay General Hospital, Taipei 10630, Taiwan.

Polymers
|January 16, 2020
PubMed
Summary
This summary is machine-generated.

Applying an external electric field to polyelectrolyte multilayers (PEMs) containing DNA and polyethylenimine (PEI) enhances gene delivery. This pretreatment effectively releases gene vectors for improved transfection, benefiting regenerative medicine.

Keywords:
electrical fieldgene deliverylayer-by-layer assemblypolyelectrolyte multilayerpolypyrrole

More Related Videos

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

8.9K
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

16.4K

Related Experiment Videos

Last Updated: Dec 31, 2025

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
04:46

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

Published on: September 12, 2011

10.7K
Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

8.9K
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

16.4K

Area of Science:

  • Biomaterials Science
  • Gene Delivery Systems
  • Nanotechnology

Background:

  • Layer-by-layer (LbL) assembly creates polyelectrolyte multilayers (PEMs) for gene delivery, encapsulating plasmid DNA and cationic nonviral vectors like polyethylenimine (PEI).
  • Efficient release of these macromolecules from PEMs for effective transfection remains a significant challenge due to macromolecular entanglement.

Purpose of the Study:

  • To investigate the use of external electric field pretreatment to enhance the release of DNA and PEI from PEMs.
  • To evaluate the impact of electric field parameters (voltage, time) on release efficiency and subsequent transfection.

Main Methods:

  • Fabrication of PEMs composed of PEI and DNA on a conductive polypyrrole (PPy) substrate.
  • Application of a perpendicular electric field across the PEMs in an aqueous environment.
  • Analysis of PEI and DNA release, PEM surface roughness changes, and polyplex formation post-treatment.
  • Assessment of transfection efficiency using the released gene vectors.

Main Results:

  • An electric field perpendicular to the substrate efficiently promoted PEI and DNA release from PEMs.
  • Higher electric potential and longer treatment times resulted in greater macromolecule release.
  • Electric field treatment increased PEM roughness due to electrophoresis and electrochemical reactions on the PPy electrode.
  • Released DNA and PEI formed polyplexes, leading to improved transfection efficiency.

Conclusions:

  • External electric field pretreatment is an effective method for facilitating gene delivery from PEMs.
  • This approach enhances the release of gene vectors and improves transfection efficiency.
  • The findings suggest significant potential for electric field-assisted gene delivery in regenerative medicine applications.