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Determination of Polyhexamethylene Biguanide Hydrochloride Using a Lactone-Rhodamine B-Based Fluorescence Optode
Akane Funaki1, Yuta Horikoshi1, Teruyuki Kobayashi1
1Department of Applied Chemistry, Faculty of Engineering, Shibaura Institute of Technology, Toyosu, Koto-ku, Tokyo 135-8548, Japan.
Molecules (Basel, Switzerland)
|January 16, 2020
Summary
A novel fluorescence optode method accurately determines polyhexamethylene biguanide hydrochloride (PHMB) in solutions. This sensitive technique offers a reliable way to quantify PHMB, crucial for disinfectant applications.
Area of Science:
- Analytical Chemistry
- Chemical Sensing
- Materials Science
Background:
- Polyhexamethylene biguanide hydrochloride (PHMB) is a widely used disinfectant.
- Accurate determination methods for PHMB are essential for quality control and safety.
Purpose of the Study:
- To develop a new fluorescence optode method for determining PHMB concentrations.
- To validate the method's accuracy and sensitivity for PHMB detection.
Main Methods:
- Fabrication of an optode membrane using lactone-rhodamine B (L-RB), poly (vinyl chloride), and 2-nitrophenyl octyl ether.
- Utilizing tetrakis (4-fluorophenyl) borate, sodium salt (NaTPBF) as a responsive agent.
- Measuring the decrease in L-RB fluorescence intensity correlated with PHMB concentration.
Main Results:
- The optode demonstrated sensitivity to NaTPBF in the μM range.
- A linear decrease in fluorescence intensity was observed for PHMB concentrations from 0 to 8.0 μM.
- The method accurately determined PHMB concentrations in contact lens detergents, aligning with nominal values.
Conclusions:
- The developed L-RB based fluorescence optode provides a reliable method for PHMB determination.
- This technique is suitable for quantifying PHMB in relevant commercial products like contact lens solutions.
