Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hypoxia

Hypoxia

Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

3-(4-Hydroxy-3-methoxyphenyl) propionic acid prevents renal fibrosis and inflammation in CKD mice induced by adenine.

Scientific reports·2026

Same author

CRISPR-Cas9 disruption of flavanone 3-hydroxylase produces a green phenotype and alters flavone metabolites in allotetraploid perilla.

Frontiers in plant science·2026

Same author

Genome-Wide Identification of Transcriptional Start Sites and Candidate Enhancers Regulating Worker Metamorphosis in <i>Apis mellifera</i>.

Insects·2026

Same author

Workflow for Fine-Tuning and Evaluating DNA Language Models for Specific Genomics Issues.

Bio-protocol·2026

Same author

Hypoxia and aspirin additively increase intracellular glutamine accumulation in PIK3CA-mutated colorectal cancer cells.

Scientific reports·2026

Same author

Genome assembly and annotation of the naked mole rat Heterocephalus glaber reared in Japan.

Scientific data·2026

Search research articles

Related Experiment Video

Updated: Dec 31, 2025

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Published on: August 10, 2017

Meta-Analysis of Hypoxic Transcriptomes from Public Databases.

Hidemasa Bono¹, Kiichi Hirota²

¹Database Center for Life Science (DBCLS), Joint Support-Center for Data Science Research, Research Organization of Information and Systems, 1111 Yata, Mishima, Shizuoka 411-8540, Japan.

|January 16, 2020

Summary

This summary is machine-generated.

This study analyzed RNA-seq data to understand cellular responses to low oxygen (hypoxia). It identified common hypoxia-responsive genes in humans and mice, offering a valuable resource for hypoxia research.

Keywords:

ChIP-seq RNA-seq hypoxia meta-analysis public database transcriptome

More Related Videos

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Published on: May 13, 2014

Related Experiment Videos

Last Updated: Dec 31, 2025

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Published on: August 10, 2017

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Published on: May 13, 2014

Area of Science:

Cellular Biology
Molecular Biology
Genomics

Background:

Hypoxia, or cellular oxygen insufficiency, is regulated by hypoxia-inducible factors (HIFs).
Understanding the cellular response to hypoxia is crucial for various biological processes and diseases.

Purpose of the Study:

To perform a data-driven meta-analysis of RNA-seq data to gain functional insights into the hypoxic response.
To identify common hypoxia-responsive genes between humans and mice.
To explore hypoxia-responsive pathways involving direct transcription factor binding by integrating transcriptome data with HIF ChIP-seq data.

Main Methods:

Manual curation of 128 human and 52 murine RNA-seq transcriptome pairs before and after hypoxic stress.
Classification of gene expression changes (upregulated, downregulated, unchanged) for each gene.
Meta-analysis of hypoxic transcriptomes and integration with public ChIP-seq data for HIF-1 and HIF-2.

Main Results:

Identification of common hypoxia-responsive genes between human and mouse transcriptomes.
Insights into hypoxia-responsive pathways driven by direct transcription factor binding of HIFs.
Demonstration of meta-analysis utility for candidate gene selection from diverse gene expression datasets.

Conclusions:

The study provides a valuable resource for the scientific community researching hypoxia.
Meta-analysis of public gene expression databases is a powerful approach for identifying candidate genes under various experimental conditions.