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LINC00908 negatively regulates microRNA-483-5p to increase TSPYL5 expression and inhibit the development of prostate

Li Fan1, Hai Li1, Yun Zhang1

  • 1Department of Urology, China-Japan Union Hospital of Jilin University, No. 126, Xiantai Street, Changchun, 130033 Jilin People's Republic of China.

Cancer Cell International
|January 16, 2020
PubMed
Abstract

Insights

Long non-coding RNA LINC00908 acts as a tumor suppressor in prostate cancer (PCa) by regulating miR-483-5p and TSPYL5. Overexpression of LINC00908 inhibits PCa progression and tumor growth.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Aberrant expression of long non-coding RNAs (lncRNAs) is linked to human cancers.
  • The specific role of LINC00908 in prostate cancer (PCa) requires further investigation.

Purpose of the Study:

  • To investigate the role of LINC00908 in prostate cancer (PCa).
  • To elucidate the underlying molecular mechanisms of LINC00908 in PCa progression.

Main Methods:

  • Screening differentially expressed lncRNAs using Gene Expression Omnibus (GEO) microarray data.
  • Assessing LINC00908 expression in PCa tissues and cell lines via RT-qPCR and FISH.
  • Investigating the LINC00908/miR-483-5p/TSPYL5 interaction using bioinformatics, dual-luciferase reporter assays, RNA pull-down, RIP, and FISH.
  • Evaluating the impact of altered gene expression on PCa cell behavior and tumor growth in vivo.

Main Results:

  • LINC00908 expression was found to be downregulated in PCa tissues and cells.
  • LINC00908 competitively binds to miR-483-5p, leading to increased TSPYL5 expression.
  • Overexpression of LINC00908 suppressed PCa cell proliferation, migration, and invasion, while promoting apoptosis.
  • TSPYL5 upregulation or miR-483-5p inhibition mimicked the tumor-suppressive effects of LINC00908.
  • In vivo studies confirmed that LINC00908 overexpression inhibited PCa tumor growth.

Conclusions:

  • LINC00908 functions as a tumor suppressor in PCa by modulating the miR-483-5p/TSPYL5 axis.
  • LINC00908's ability to upregulate TSPYL5 expression inhibits PCa progression.
  • LINC00908 presents a potential novel therapeutic target for prostate cancer treatment.
Keywords:
ApoptosisInvasionLINC00908MigrationProliferationProstate cancerTSPYL5microRNA-483-5p

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