Novel Methods for High-resolution Assessment of Cardiac Action Potential Repolarization

The profile of the action potential (AP) of cardiomyocytes contributes to the modality of ventricular repolarization of the heart. Experimentally, the examination of the AP in isolated cardiomyocytes provides information on their electrical properties, adaptations to physiological and pathological conditions, and putative ionic mechanisms involved in the process. Currently, there are no available platforms for automated assessment of AP properties and standard methodologies restrict the examination of the AP repolarization to discrete, user-defined ranges, neglecting significant intervals of the electrical recovery. This study proposes two automatic methods to assess AP profile throughout the entire repolarization phase. One method is based on AP data inversion and direct extraction of patterns describing beat-to-beat dynamics. The second method is based on evolutive singular value decomposition (ESVD), which identifies common patterns in a series of consecutive APs. The two methodologies were employed to analyze electrical signals collected from cardiomyocites obtained from healthy mice and animals with diabetes, a condition associated with alterations of AP properties in cardiac cells. Our methodologies revealed that the duration of the early repolarization phase of the AP tended to become progressively longer during a stimulation train, whereas the late repolarization progressively shortened. Although this behavior was comparable in the two groups of cells, alterations in AP dynamics occurred at distinct repolarization levels, a feature highlighted by the ESVD approach. In conclusion, the proposed methodologies allow detailed, automatic analysis of the AP repolarization and identification of critical alterations occurring in the electrical behavior of myocytes under pathological conditions.