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Published on: August 8, 2014
An experimental hyperlipidemia model with periodontitis in mice
Xiaoxuan Liu1, Zhiguo Wang2, Qiuxia Ji1
1Department of Stomatology, The Affiliated Hospital of Qingdao University, College of Stomatology, Qingdao University Qingdao 266003, Shandong, China.
Purpose:
In many animal models and clinical trials, the relationship between periodontitis and hyperlipidemia is bidirectional and interlinked. In this study, an experimental hyperlipidemia model with periodontitis in mice is introduced.
Methods:
C57BL/6J mice were assigned into group A and B and Apolipoprotein E-deficient (ApoE-/-) mice into group C. After 4 weeks of a high fat diet (HFD), group B and C were ligated on the maxillary second molar tooth, and mice were sacrificed after 8 weeks of the HFD. Levels of lipids, interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α in serum after 0, 4, and 8 weeks were determined. Alveolar bone loss (ABL) was assessed under stereomicroscope. Maxillary bones and atherosclerotic lesion area in the aorta were collected for hematoxylin and eosin (HE) staining.
Results:
After feeding with a HFD for 4 weeks, group C demonstrated dramatic increases in serum lipid levels. The ABL and levels of IL-6, IL-10, and TNF-α in group C was significantly higher than those of group A and B (P<0.05). Atherosclerotic lesions were observed in group C.
Conclusions:
These data demonstrate that an experimental hyperlipidemia model with periodontitis in mice is successfully established by ligation in ApoE-/- mice. This method is economical and time saving, and worthy of more general application.
