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GRP78 overexpression as an unfavorable outcome in glioma patients
Lujun Bao1, Qisheng Luo2,3, Junyi Zhang2
1Zhongshan Chenxinghai Hospital, Guangdong Medical University Zhongshan, China.
Aims:
In this study, the GRP78 expression and the correlation between GRP78 expression and clinicopathologic data in patients with glioma, including survival, were examined.
Methods And Results:
The mRNA and protein levels of GRP78were respectively determined by real-time PCR and immunohistochemical analysis in 30 fresh glioma samples and 19 fresh normal brain samples as well as 156 paraffin-embedded glioma samples and 35 normal paraffin-embedded brain samples. The data showed that GRP78 mRNA is markedly upregulated compared with normal brain tissues. Consistent with this data, the GRP78 protein level was also significantly increased in glioma tissues compared with normal brain tissues. We further observed that high GRP78 protein expression was significantly associated with clinical stage (P = 0.0013) but did not correlate with age and gender. High, rather than low, GRP78 protein expression was associated with pooroverall survival rates (P = 0.001). Multivariate analysis indicated that high GRP78 protein expression was an independent prognostic indicator of patient survival (P = 0.002).
Conclusions:
Our findings demonstrate that GRP78 is overexpressed and plays a significant role in disease progression and poor outcome in patients with glioma.
