Expression of Wnt/β-catenin related genes after skeletal muscle contusion

It was aimed to determine expressions of genes related to Wnt/β-catenin signaling for evaluating time duration after skeletal muscle contusion.

Pathological change of skeletal muscle was observed after H-E staining. mRNA of respective genes was quantified with real-time quantitative PCR. Expression of β-catenin was further characterized with immunostaining and quantified as intensity/area and further immune blotting and quantified as grey intensity normalized to loading control (GADPH).

After injury, skeletal muscle exhibited prominent inflammatory response, hyperplasia and regeneration. Infiltration of inflammatory cell, formation of myotube and maturation of skeletal muscle fiber were observed under HE staining. Expression of FZD4, Myo D, Myf5 changed during early stages after injury and could serve to evaluate injury within 24 h; Expression of SFRP5 and Fra1 changed during early-to-intermediate stages after injury and could serve to evaluate injury within 12-48 h; Expression of MRF4 changed during intermediate stages after injury and could serve to evaluate injury within 36-48 h; Expression of β-catenin changed during intermediate stages after injury and could serve to evaluate injury within 36 h-3 d; Expression of MyoG changed during late stages after injury and could serve to evaluate injury within 48 h-7 d. Immunostaining experiments showed that 36 h after injury, membrane β-catenin decreased while nucleus β-catenin increased.

Wnt/β-catenin related genes are involved in regeneration of skeletal muscle after contusion. The sequential changes of gene expression can be used for evaluating the duration after contusion.