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MicroRNA-128 increases glioma cell radio-sensitivity by suppressing senescent evasion through oncogene Bmi-1

Junhua Sun1, Lan Ye1, Cuihong Wang1

  • 1Cancer Center, The Second Hospital of Shandong University Jinan, Shandong, P. R. China.

International Journal of Clinical and Experimental Pathology
|January 16, 2020
PubMed

Insights

Overexpressing microRNA-128 in glioblastoma (GBM) cells inhibits growth and Bmi-1 expression. This enhances radio-sensitivity, offering a new strategy against radio-resistant brain tumors.

Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Radiation Oncology

Background:

  • Glioblastoma (GBM) is an aggressive brain tumor with challenging radio-resistance.
  • MicroRNA-128 (miR-128) is downregulated in gliomas, while its target Bmi-1 promotes glioma growth and radio-resistance.
  • The mechanism by which Bmi-1 confers radio-resistance remains unclear.

Purpose of the Study:

  • To investigate the role of microRNA-128 in glioblastoma radio-resistance.
  • To elucidate the mechanism involving Bmi-1 and cellular senescence.

Main Methods:

  • Overexpression of microRNA-128 in glioma cells.
  • Exposure to 8 Gy X-ray radiation.
  • Assessment of cell growth, Bmi-1 expression, and senescence.

Main Results:

  • MicroRNA-128 overexpression inhibited glioma cell growth and Bmi-1 expression.
  • Radiation exposure showed reduced cell growth in miR-128 overexpressing cells.
  • Lower Bmi-1 expression and increased senescence were observed in the miR-128 overexpression group.

Conclusions:

  • Overexpression of microRNA-128 enhances glioma cell radio-sensitivity by downregulating Bmi-1.
  • This mechanism may involve inhibiting senescent evasion, providing a novel strategy for glioma radiation therapy.
Keywords:
Bmi-1GliomamicroRNA-128radiationsenescence

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