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miR-182 promotes cell proliferation and invasion by inhibiting APC in melanoma

Xilin Liu1, Hong Li2, Guangzhi Wu1

  • 1Department of Hand Surgery, China-Japan Union Hospital of Jilin University Changchun, Jilin Province, China.

International Journal of Clinical and Experimental Pathology
|January 16, 2020
PubMed
Abstract

Insights

MicroRNA-182 (miR-182) is overexpressed in melanoma, promoting cancer cell proliferation and invasion. Targeting miR-182 and APC may offer new therapeutic strategies for melanoma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Melanoma treatment faces challenges due to limited therapeutic effects.
  • The precise pathogenesis of melanoma remains unclear, necessitating novel treatment strategies.
  • MicroRNA-182 (miR-182) is significantly overexpressed in human melanoma.

Purpose of the Study:

  • To investigate the role and underlying mechanism of miR-182 in melanoma.
  • To explore miR-182 as a potential therapeutic target for melanoma.

Main Methods:

  • RT-PCR was used to detect miR-182 expression in melanoma tissues and cell lines.
  • Cell proliferation, apoptosis, invasion, and cell cycle were assessed using CCK-8 assays.
  • Western blotting analyzed protein expression of key molecules in the Wnt/β-catenin pathway.

Main Results:

  • miR-182 expression was significantly upregulated in melanoma.
  • Overexpression of miR-182 enhanced melanoma cell proliferation, migration, and invasion.
  • miR-182 targeted APC, influencing proliferation and tumor growth.

Conclusions:

  • Overexpression of miR-182 promotes melanoma cell proliferation and invasion by targeting APC.
  • The miR-182 and APC pathway presents a potential therapeutic target for melanoma treatment at the molecular level.
Keywords:
APCapoptosismelanomamiR-182proliferation

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