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Updated: Dec 30, 2025

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
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ATP-binding affinity of the ε subunit of thermophilic F1-ATPase under label-free conditions

Miria Fujiwara1, Yasuyuki Kato-Yamada1,2

  • 1Department of Life Science, College of Science, Rikkyo University, Japan.

Biochemistry and Biophysics Reports
|January 16, 2020
PubMed

Keywords:
ATP bindingATP synthaseBiosensorInhibitorKd-ATP, dissociation constant of ε subunit for ATPKd-Cy3, dissociation constant of Cy3-labeled ε subunit for ATPKd-Mant, dissociation constant of ε subunit for Mant-ATPLabel freeMant-ATP, 2′(3′)-O-N′-methylaniloyl-aminoadenosine-5′-triphosphateTF1, F1-ATPase from Bacillus sp. PS3

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