Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Patch Clamp01:18

Patch Clamp

6.2K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
6.2K
Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

530
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
530
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

7.0K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sustainable Biosynthesis of Fludarabine by a Novel Mixed Nanostabilized Biocatalyst.

Applied biochemistry and biotechnology·2025
Same author

Dihydroxyacetone production via heterogeneous biotransformations of crude glycerol.

Journal of biotechnology·2021
Same author

Decitabine bioproduction using a biocatalyst with improved stability by adding nanocomposites.

AMB Express·2020
Same author

Biotransformation of cladribine by a nanostabilized extremophilic biocatalyst.

Journal of biotechnology·2020
Same author

Hyperstabilization of a thermophile bacterial laccase and its application for industrial dyes degradation.

3 Biotech·2020
Same author

Bioproduction of ribavirin by green microbial biotransformation.

Process biochemistry (Barking, London, England)·2020

Related Experiment Video

Updated: Dec 30, 2025

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

5.4K

Whole Cell Entrapment Techniques.

Jorge A Trelles1, Cintia W Rivero2

  • 1Laboratorio de Investigaciones en Biotecnología Sustentable (LIBioS), Universidad Nacional de Quilmes, Bernal, Argentina. jtrelles@unq.edu.ar.

Methods in Molecular Biology (Clifton, N.J.)
|January 16, 2020
PubMed
Summary

Microbial whole cell immobilization, particularly cell entrapment, offers an efficient, stable, and scalable method for bioprocessing. This technique enhances catalyst performance and simplifies separation in industrial applications.

Keywords:
AgarAgaroseAlginateEntrapment immobilizationPolyacrylamidePost-immobilizationReusabilityStabilityWhole cells

More Related Videos

High Throughput Single-cell and Multiple-cell Micro-encapsulation
16:19

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

19.1K
Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel
10:20

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

Published on: June 29, 2017

20.4K

Related Experiment Videos

Last Updated: Dec 30, 2025

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

5.4K
High Throughput Single-cell and Multiple-cell Micro-encapsulation
16:19

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

19.1K
Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel
10:20

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

Published on: June 29, 2017

20.4K

Area of Science:

  • Biotechnology
  • Biocatalysis
  • Industrial Microbiology

Background:

  • Microbial whole cells serve as efficient, eco-friendly, and cost-effective catalysts across various industries.
  • Microorganism immobilization is crucial for optimizing bioprocesses under preparative conditions.
  • Immobilization offers benefits like enhanced operational stability, simplified separation, and scalability.

Purpose of the Study:

  • To highlight the advantages of microbial whole cell immobilization for bioprocessing.
  • To detail cell entrapment as a primary immobilization technique.
  • To emphasize the efficiency and protective benefits of cell entrapment.

Main Methods:

  • Review of microbial whole cell immobilization techniques.
  • Focus on cell entrapment within a rigid, porous network.
  • Analysis of substrate/product diffusion and cell protection.

Main Results:

  • Cell entrapment provides high immobilization efficiency, often reaching 100%.
  • The technique ensures protection of microorganisms from the reaction environment.
  • Porous networks facilitate substrate and product diffusion.

Conclusions:

  • Microorganism immobilization, especially via cell entrapment, is a highly effective strategy for industrial bioprocesses.
  • Cell entrapment offers significant advantages in terms of operational stability, separation, and scalability.
  • This method ensures efficient biocatalysis while protecting the microbial cells.