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Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
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Structure and Function of the TREX-2 Complex.

Murray Stewart1

  • 1MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0QH, UK. ms@mrc-lmb.cam.ac.uk.

Sub-Cellular Biochemistry
|January 16, 2020
PubMed
Summary
This summary is machine-generated.

The Three prime repair exonuclease 2 (TREX-2) complex aids gene expression by linking mRNA processing to nuclear pores. Structural studies reveal how TREX-2 facilitates gene activation and mRNA export.

Keywords:
ChaperoninKaryopherinNPCNuclear poreNucleoporinTREX-2 complexmRNA processing

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The Three prime repair exonuclease 2 (TREX-2) complex is crucial for nuclear mRNA processing and gene expression regulation.
  • It acts as a scaffold for mRNA processing machinery and facilitates the relocation of active genes to nuclear pores (NPCs).
  • In Saccharomyces cerevisiae, TREX-2 comprises Sac3, Thp1, Sem1, Cdc31, and Sus1 proteins.

Purpose of the Study:

  • To elucidate the structural basis of TREX-2 function in gene expression.
  • To define the roles of specific TREX-2 regions in gene activation and mRNA export.
  • To understand how TREX-2 integrates mRNA processing with nuclear pore association.

Main Methods:

  • X-ray crystallography was employed to determine the structure of key TREX-2 regions.
  • Electron microscopy provided insights into the overall complex architecture.
  • Mutagenesis studies were conducted to analyze TREX-2 function in vivo.

Main Results:

  • The structures of the nucleic acid-binding M-region and the NPC-linking CID region of TREX-2 were established.
  • Mutant analyses revealed TREX-2's contribution to positioning highly expressed genes at NPCs.
  • The complex's role in facilitating mRNA nuclear export was further defined.

Conclusions:

  • Structural insights into TREX-2 provide a foundation for understanding its multifaceted roles in gene expression.
  • TREX-2 is essential for efficient mRNA export and the spatial organization of transcription at the nuclear periphery.
  • The findings highlight TREX-2 as a key mediator between mRNA processing and nuclear pore functions.