Optogenetics: Rho GTPases Activated by Light in Living Macrophages
Maren Hülsemann1,2, Polina V Verkhusha1, Peng Guo1,2,3
1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, USA.
Abstract:
Genetically encoded optogenetic tools are increasingly popular and useful for perturbing signaling pathways with high spatial and temporal resolution in living cells. Here, we show basic procedures employed to implement optogenetics of Rho GTPases in a macrophage cell line. Methods described here are generally applicable to other genetically encoded optogenetic tools utilizing the blue-green spectrum of light for activation, designed for specific proteins and enzymatic targets important for immune cell functions.
More Related Videos
08:00Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
Published on: October 4, 2024
09:32Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
Published on: June 15, 2017
