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Updated: Dec 30, 2025
An Orthotopic Bladder Cancer Model for Gene Delivery Studies
Published on: December 1, 2013
Antiproliferative Activity of Olive Extract Rich in Polyphenols and Modified Pectin on Bladder Cancer Cells
Alejandra Bermúdez-Oria1,2, Guillermo Rodríguez-Gutiérrez1, Fátima Rubio-Senent1
1Department of Food Phytochemistry, Fat Institute (Spanish National Research Council, CSIC), Seville, Spain.
Abstract:
Bladder cancer (BC) is one of the most common human cancers. There is an interest in controlling and treating BC and other types of cancer via the use of natural substances and/or combination chemotherapy. Modified forms of pectin have been reported to possess anticancer bioactivity related to the interaction of galactosyl, a main component of pectin, with galectin-3, a carbohydrate-binding protein that is overexpressed on many types of cancer cells. In this study, the antiproliferative effect on BC of novel modified pectins extracted from olives was evaluated. Pectoliv extracts, with high polyphenol content associated to polysaccharides rich in pectin, exhibited an important antiproliferative capacity in vitro against four human BC cells lines, RT112, T24, J82, and SCaBER. Pectoliv treatment reduced the expression of galectin-1 and galectin-3 and significantly inhibited the agglutination of erythrocytes. Thus, Pectoliv may have the potential for development as a novel galectin-3 inhibitor.
Insights
Novel modified olive pectin extracts, Pectoliv, show significant antiproliferative effects against bladder cancer (BC) cells in vitro. These extracts may offer a new therapeutic strategy by inhibiting galectin-3, a protein overexpressed in many cancers.
Area of Science:
- Oncology
- Biochemistry
- Natural Product Chemistry
Background:
- Bladder cancer (BC) is a prevalent malignancy with ongoing research into novel therapeutic agents.
- Natural substances and combination chemotherapy are explored for cancer treatment.
- Modified pectins, particularly their galactosyl components, show potential anticancer activity via galectin interactions.
Purpose of the Study:
- To evaluate the antiproliferative effects of novel modified pectins from olives (Pectoliv) on human bladder cancer cell lines.
- To investigate the potential of Pectoliv as a galectin-3 inhibitor for bladder cancer therapy.
Main Methods:
- Extraction and characterization of modified pectins from olives (Pectoliv).
- In vitro antiproliferative assays against four human bladder cancer cell lines (RT112, T24, J82, SCaBER).
- Analysis of galectin-1 and galectin-3 expression levels and erythrocyte agglutination inhibition.
Main Results:
- Pectoliv extracts demonstrated significant in vitro antiproliferative capacity against all tested bladder cancer cell lines.
- Treatment with Pectoliv reduced the expression of both galectin-1 and galectin-3.
- Pectoliv significantly inhibited erythrocyte agglutination, suggesting interaction with carbohydrate-binding proteins.
Conclusions:
- Novel modified olive pectin extracts (Pectoliv) possess notable antiproliferative properties against bladder cancer cells.
- Pectoliv's mechanism may involve the inhibition of galectin-3, a key protein in cancer progression.
- Pectoliv shows potential as a natural compound for developing new galectin-3 inhibitors for bladder cancer treatment.
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