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Updated: Dec 30, 2025

Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging
Published on: July 11, 2025
Transient absorption microscopy: Technological innovations and applications in materials science and life science
1Department of Chemistry, Boston University, Boston, Massachusetts 02215, USA.
Abstract:
Transient absorption (TA) spectroscopy has been extensively used in the study of excited state dynamics of various materials and molecules. The transition from TA spectroscopy to TA microscopy, which enables the space-resolved measurement of TA, is opening new investigations toward a more complete picture of excited state dynamics in functional materials, as well as the mapping of crucial biopigments for precision diagnosis. Here, we review the recent instrumental advancement that is pushing the limit of spatial resolution, detection sensitivity, and imaging speed. We further highlight the emerging application in materials science and life science.
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