Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Alpha-mannosidase in human red cells.

L Poenaru, J C Dreyfus

    Biochimica Et Biophysica Acta
    |January 12, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Red blood cells contain alpha-mannosidase, neutral alpha-glucosidase, and beta-hexosaminidase enzymes. Red cell alpha-mannosidase differs biochemically and genetically from lysosomal acid mannosidase.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Metabolism of muscle proteins].

    Progress in nuclear energy. Series 6 Biological sciences·2014
    Same author

    [Metabolic heterogeneity of hemoglobin].

    Progress in nuclear energy. Series 6 Biological sciences·2014
    Same author

    Sensitivity of the bile ducts to variations in pH.

    Archives des maladies de l'appareil digestif et des maladies de la nutrition·2010
    Same author

    On a new fraction of muscle iron.

    Comptes rendus des seances de la Societe de biologie et de ses filiales·2010
    Same author

    Iron and muscle wasting.

    Comptes rendus des seances de la Societe de biologie et de ses filiales·2010
    Same author

    Myoglobin (muscular hemoglobin) has its place in the normal and pathological biochemistry of the muscle.

    La semaine des hopitaux : organe fonde par l'Association d'enseignement medical des hopitaux de Paris·2010
    Same journal

    Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Molecular Basis of Disease Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    General Subjects Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

    Biochimica et biophysica acta·2019
    Same journal

    Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

    Biochimica et biophysica acta·2019
    Same journal

    Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

    Biochimica et biophysica acta·2019
    See all related articles

    Area of Science:

    • Biochemistry
    • Enzymology
    • Cell Biology

    Background:

    • Lysosomal hydrolases are crucial for cellular waste breakdown.
    • Investigating enzyme activity in red blood cells provides insights into cellular function and potential disease markers.

    Purpose of the Study:

    • To identify lysosomal hydrolases and related enzymes in human and rabbit red blood cell hemolysates.
    • To characterize the properties of alpha-mannosidase found in red blood cells.

    Main Methods:

    • Hemolysates from human and rabbit red blood cells were analyzed for enzyme activities.
    • Alpha-mannosidase activity was quantified, and its optimal pH and electrophoretic pattern were determined.
    • Enzyme characteristics were compared between red blood cells and white blood cells, and in patients with mannosidosis.

    Related Experiment Videos

    Main Results:

    • Significant activities of alpha-mannosidase, neutral alpha-glucosidase, and beta-hexosaminidase were detected in red cell hemolysates.
    • Red blood cell alpha-mannosidase exhibited approximately 200-fold lower activity per cell compared to white blood cells.
    • Optimal pH for red cell alpha-mannosidase was determined to be 5.5-6.0, with three distinct electrophoretic bands observed.
    • Patients with mannosidosis did not show a deficiency in red cell alpha-mannosidase, and enzyme properties were similar to controls.

    Conclusions:

    • Red blood cells possess specific enzymatic activities, including alpha-mannosidase, neutral alpha-glucosidase, and beta-hexosaminidase.
    • The alpha-mannosidase identified in red blood cells exhibits distinct biochemical and genetic properties compared to the lysosomal acid form.
    • These findings suggest that red cell alpha-mannosidase is a distinct enzyme entity, not directly related to the lysosomal enzyme deficient in mannosidosis.