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Aldehyde dehydrogenase 2 inhibition potentiates 4-hydroxy-2-nonenal induced decrease in angiogenesis of coronary

Bipradas Roy1,2, Suresh Selvaraj Palaniyandi1,2

  • 1Division of Hypertension and Vascular Research, Department of Internal Medicine, Henry Ford Health System, Detroit, Michigan.

Cell Biochemistry and Function
|January 17, 2020
PubMed
Summary

Aldehyde dehydrogenase 2 (ALDH2) protects against 4-hydroxynonenal (4HNE)-induced damage. ALDH2 inhibition worsens 4HNE

Keywords:
4-hydroxy-2-nonenalERGSirtuin 1VEGFaldehyde dehydrogenase 2angiogenesiscoronary endothelial cells

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Area of Science:

  • Cardiovascular Biology
  • Cellular and Molecular Medicine
  • Biochemistry

Background:

  • Coronary endothelial cell (EC) dysfunction and impaired angiogenesis are hallmarks of cardiac diseases.
  • 4-Hydroxynonenal (4HNE), a product of lipid peroxidation, accumulates in cardiac disease and causes cellular toxicity.
  • Aldehyde dehydrogenase 2 (ALDH2) is a key enzyme metabolizing 4HNE and mitigating its cytotoxic effects.

Purpose of the Study:

  • To investigate the role of ALDH2 in regulating 4HNE-induced effects on coronary EC angiogenesis.
  • To test the hypothesis that ALDH2 inhibition potentiates 4HNE-mediated reduction in coronary EC angiogenesis in vitro.

Main Methods:

  • Cultured mouse coronary EC (MCEC) were treated with varying concentrations of 4HNE.
  • ALDH2 was pharmacologically inhibited using disulfiram (DSF) prior to 4HNE challenge.
  • Angiogenesis was assessed by tube formation assays, and the expression of key proteins (VEGFR2, SIRT1, ERG) was quantified via mRNA and protein analysis.

Main Results:

  • 4HNE treatment alone attenuated MCEC tube formation, indicating reduced angiogenesis.
  • 4HNE significantly downregulated VEGFR2, SIRT1, and ERG expression.
  • ALDH2 inhibition by DSF potentiated the 4HNE-induced decrease in angiogenesis and further reduced VEGFR2, SIRT1, and ERG expression compared to 4HNE alone.

Conclusions:

  • ALDH2 plays a protective role in coronary ECs by counteracting the antiangiogenic effects of 4HNE.
  • ALDH2 functions as a proangiogenic signaling molecule by alleviating 4HNE-induced impairment of angiogenesis.
  • Targeting ALDH2 may offer a therapeutic strategy for cardiac diseases associated with EC dysfunction and impaired angiogenesis.