Statistical analysis of the crystallinity index of nanocellulose produced from Kraft pulp via controlled enzymatic
Ruan Stevan Almeida Ribeiro1, Ninoska Bojorge1, Nei Pereira2
1Department of Chemical and Petroleum Engineering, Fluminense Federal University, Niterói, RJ, Brazil.
Abstract:
Enzymatic hydrolysis processes can change the physical characteristics of nanocellulose derived from Kraft pulp. Among these attributes are its crystallinity index and dimensions. In this study, we determined the optimal conditions under which nanocellulose could be produced enzymatically with the greatest increase of the crystallinity index relative to its initial state. Application of Central Composite Rotatable Design statistical analysis to the experiments was employed to direct an increase the crystallinity index in 10% at the 24-H hydrolysis time. Upon establishment of ideal levels of starting material and enzyme, reactions were carried out at hydrolysis times of 24, 48, and 72 H under these ideal parameters. The effectiveness of deagglomeration was demonstrated by measuring the hydrodynamic diameter of the particles by dynamic light scattering. Scanning electron microscopy was performed on four samples, the original material, kraft pulp, and hydrolyzed biomaterials at 72 H in the ideal parameters. The hydrolyzed material with the best statistical data, revealing a fiber diameter of 180 nm, disclosing to be biomaterial with nanocellulose dimensions.
