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Updated: Dec 30, 2025

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
Published on: January 22, 2018
Structure Validation of G-Rich RNAs in Noncoding Regions of the Human Genome
Oliver Binas1, Irene Bessi2, Harald Schwalbe1
1Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Max-von-Laue Strasse 7, 60438, Frankfurt, Germany.
Abstract:
We present the rapid biophysical characterization of six previously reported putative G-quadruplex-forming RNAs from the 5'-untranslated region (5'-UTR) of silvestrol-sensitive transcripts for investigation of their secondary structures. By NMR and CD spectroscopic analysis, we found that only a single sequence-[AGG]2 [CGG]2 C-folds into a single well-defined G-quadruplex structure. Sequences with longer poly-G strands form unspecific aggregates, whereas CGG-repeat-containing sequences exhibit a temperature-dependent equilibrium between a hairpin and a G-quadruplex structure. The applied experimental strategy is fast and provides robust readout for G-quadruplex-forming capacities of RNA oligomers.
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