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Updated: Dec 30, 2025

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores
Published on: April 5, 2022
A colorimetric probe for detection of Cu2+ by the naked eye and application in test paper
Xiaorui Cao1, Qi Gao1, Xiaotao He1
1Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi, China.
Abstract:
A novel colorimetric probe RP1 was synthesized using rhodamine derivatives and heterocyclic compounds for the purpose of detecting Cu2+ . RP1 showed good selectivity, high sensitivity and affinity toward Cu2+ over other competing ions in CH3 OH-H2 O (1/1, v/v) solution. Absorbance intensity showed a good linear fit between probe R1 and Cu2+ over the concentration range 1-8 μM and the association constant was also calculated to be 1.145 × 105 M-1 . The sensing mechanism was deduced using Job's plot, Fourier transform infrared spectroscopy, and density functional theory studies. In addition, the colorimetric experiment indicated that probe RP1 could be made into test paper to detect Cu2+ with a colour change from colourless to pink.
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