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Thinking beyond tradition: Polyphenols as effective refolding modulators.

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PubMed
Summary

Polyphenols like EGCG and baicalein, combined with beta cyclodextrin, enhance protein refolding yields. This approach effectively increases the recovery of aggregation-prone proteins, such as TGFβ3.

Keywords:
Beta cyclodextrinDisaggregationPolyphenolsRefoldingTGFβ3

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Area of Science:

  • Biochemistry and Molecular Biology
  • Protein Chemistry and Engineering

Background:

  • Protein aggregation is a major challenge in refolding, limiting yields of complex proteins like TGFβ3.
  • Polyphenols are known amyloid alleviators but are underutilized in protein refolding/renaturation processes.

Purpose of the Study:

  • To investigate the efficacy of polyphenols, alone and with beta cyclodextrin, in improving the refolding yield of aggregation-prone proteins.
  • To explore the mechanisms by which polyphenols influence protein refolding and aggregation kinetics.

Main Methods:

  • Refolding experiments using TGFβ3 in the presence of polyphenols (EGCG, baicalein, myricetin) and beta cyclodextrin.
  • Analysis of refolding yield and kinetics using non-reducing SDS-PAGE and size exclusion chromatography.
  • Mechanism elucidation via Isothermal Titration Calorimetry (ITC) and assessment of aggregation kinetics and protein stability.

Main Results:

  • Refolding with baicalein or EGCG combined with beta cyclodextrin significantly increased native TGFβ3 yield in a time-dependent manner.
  • EGCG accelerated refolding, achieving maximum yield increase within 24 hours, while baicalein required 48 hours.
  • Polyphenols were shown to influence aggregation kinetics and stability of native TGFβ3.

Conclusions:

  • Polyphenols, particularly EGCG and baicalein, in conjunction with beta cyclodextrin, represent a promising strategy to enhance refolding yields of aggregation-prone proteins.
  • This approach offers a novel and effective alternative for improving protein refolding efficiency in biotechnological applications.