In vitro quantification of lactate in Phosphate Buffer Saline (PBS) samples

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Spectroscopy

Background:

• Continuous lactate monitoring is vital for intensive care patients at risk of sepsis.
• Current methods rely on invasive arterial blood sampling, lacking continuous real-time data.
• There is a critical need for noninvasive lactate measurement tools in clinical settings.

Purpose of the Study:

• To investigate the potential of Near-Infrared (NIR) spectroscopy for noninvasive blood lactate concentration prediction.
• To establish a method for real-time, continuous lactate monitoring using spectral data.
• To develop and validate multivariate models for accurate lactate quantification.

Main Methods:

• Collected NIR spectra (800-2600 nm) from 37 phosphate-buffered saline samples with varying sodium lactate concentrations (0-20 mmol/L).
• Employed Partial Least-Squares (PLS) regression analysis for spectral data calibration.
• Utilized leave-one-out cross-validation to assess model performance and accuracy.

Main Results:

• Achieved a high correlation coefficient (0.977) between predicted and prepared lactate concentrations.
• Reported a low standard error of 0.89 mmol/L, indicating precise lactate quantification.
• Demonstrated the feasibility of predicting sodium lactate concentrations from NIR spectra.

Conclusions:

• NIR spectroscopy, coupled with multivariate modeling, offers a viable noninvasive approach for blood lactate assessment.
• This technology could significantly advance patient monitoring in intensive care units.
• Further research may lead to a practical, noninvasive tool for continuous lactate level monitoring.