An integrated and automated electronic system for point-of-care protein testing

Protein testing in blood is important for clinical analysis. Traditional blood tests are performed in centralized laboratories and are slow to provide results. In contrast, point-of-care devices deliver rapid results in non-laboratory settings, allowing timely analysis and in turn reducing healthcare costs. Successful point-of-care platforms require seamless integration of chemical assays, fluid management and signal readout. In this regard, we present an integrated, compact and automated electronic system for point-of-care protein sensing. The system comprises of a microfluidics-based electrochemical biosensor, amperometry circuitry and automated microfluidic fluid handling circuitry. This platform utilizes magnetic microbeads to expedite an electronic enzyme-linked immunosorbent assay, and microfluidics to manage small volumes and automate assay operations. A commercial single-chip potentiostat is utilized for amperometry measurements and microfluidics control. Using this all-electrical system, we demonstrate an integrated and automated assay for human interleukin-6.