Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells

Area of Science:

• Oncology
• Pharmacology
• Cell Biology

Background:

• Spices exhibit antioxidant, anti-inflammatory, immunomodulatory, and anticancer properties.
• Mechanisms include apoptosis induction and inhibition of tumor proliferation, migration, and invasion.
• Spices can sensitize tumors to radiotherapy and chemotherapy.

Purpose of the Study:

• To investigate the anticancer potential of clove buds extract (CBE).
• To analyze CBE's ability to induce oxidative stress and DNA damage in cancer cells.
• To examine CBE's modulation of stress, survival, and apoptotic pathways.

Main Methods:

• MCF-7 breast cancer cells were treated with CBE.
• Assessed oxidative stress markers, including oxygen and nitrogen radicals.
• Analyzed DNA damage, mitochondrial factors, and key signaling pathways (Akt, MAPK, Erk).

Main Results:

• CBE treatment induced intrinsic caspase-dependent cell death.
• Increased oxidative stress and release of mitochondrial pro-apoptotic factors were observed.
• CBE modulated the antioxidant superoxide dismutase (SOD) system and key signaling pathways.

Conclusions:

• CBE exhibits anticancer effects by inducing oxidative stress and apoptosis in MCF-7 cells.
• CBE disrupts cancer cell survival pathways and enhances DNA damage.
• Clove extract demonstrates potential as a therapeutic agent in cancer treatment.