Related Experiment Video
Updated: Dec 30, 2025

Development of a Cabbage Protoplast System for Studying Hypoxia Tolerance in Brassica
Published on: September 20, 2024
Exogenously Applied Ascorbic Acid-Mediated Changes in Osmoprotection and Oxidative Defense System Enhanced Water
Ayesha Farooq1, Shazia Anwer Bukhari2, Nudrat A Akram2
1Department of Biochemistry, Government College University, Faisalabad 38040, Pakistan.
Abstract:
The present study was conducted to examine the effect of exogenously applied ascorbic acid (AsA) on osmoprotectants and the oxidative defense system in four cultivars (16171, 16183, 16207 and 16246) of safflower under well-watered and water deficit conditions. Water stress (60% field capacity) significantly decreased the shoot and root fresh and dry weights, shoot and root lengths and chlorophyll contents in all four safflower cultivars, while it increased the leaf free proline, total phenolics, total soluble proteins, hydrogen peroxide content and activities of catalase, superoxide dismutase and peroxidase enzymes. Foliar-applied (100 mg L-1 and 150 mg L-1) ascorbic acid caused a marked improvement in shoot and root fresh and dry weights, plant height, chlorophyll and AsA contents as well as the activity of peroxidase (POD) enzyme particularly under water deficit conditions. It also increased the accumulation of leaf proline, total phenolics, total soluble proteins and glycine betaine (GB) content in all four cultivars. Exogenously applied AsA lowered the contents of MDA and H2O2, and the activities of CAT and SOD enzymes. Overall, exogenously applied AsA had a positive effect on the growth of safflower plants under water deficit conditions which could be related to AsA-induced enhanced osmoprotection and regulation of antioxidant defense system.
More Related Videos
Related Concept Videos
Adaptations that Reduce Water Loss
Responses to Salt Stress
Responses to Heat and Cold Stress
Responses to Drought and Flooding
Tonicity in Plants
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
Tonicity in Plants

